Complete Lipooligosaccharide Structure from <i>Pseudoalteromonas nigrifaciens</i> Sq02-Rif<sup>r</sup> and Study of Its Immunomodulatory Activity
Rossella Di Guida,
Angela Casillo,
Antonietta Stellavato,
Celeste Di Meo,
Soichiro Kawai,
Jun Kawamoto,
Takuya Ogawa,
Tatsuo Kurihara,
Chiara Schiraldi,
Maria Michela Corsaro
Affiliations
Rossella Di Guida
Department of Chemical Sciences, University of Naples “Federico II”, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, Italy
Angela Casillo
Department of Chemical Sciences, University of Naples “Federico II”, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, Italy
Antonietta Stellavato
Department of Experimental Medicine, Section of Biotechnology Medical Histology and Molecular Biology, University of Campania “Luigi Vanvitelli”, Via L. De Crecchio n 7, 80138 Naples, Italy
Celeste Di Meo
Department of Experimental Medicine, Section of Biotechnology Medical Histology and Molecular Biology, University of Campania “Luigi Vanvitelli”, Via L. De Crecchio n 7, 80138 Naples, Italy
Soichiro Kawai
Institute for Chemical Research, Kyoto University, Kyoto 611-0011, Japan
Jun Kawamoto
Institute for Chemical Research, Kyoto University, Kyoto 611-0011, Japan
Takuya Ogawa
Institute for Chemical Research, Kyoto University, Kyoto 611-0011, Japan
Tatsuo Kurihara
Institute for Chemical Research, Kyoto University, Kyoto 611-0011, Japan
Chiara Schiraldi
Department of Experimental Medicine, Section of Biotechnology Medical Histology and Molecular Biology, University of Campania “Luigi Vanvitelli”, Via L. De Crecchio n 7, 80138 Naples, Italy
Maria Michela Corsaro
Department of Chemical Sciences, University of Naples “Federico II”, Complesso Universitario Monte S. Angelo, Via Cintia 4, 80126 Naples, Italy
Lipopolysaccharides (LPS) are surface glycoconjugates embedded in the external leaflet of the outer membrane (OM) of the Gram-negative bacteria. They consist of three regions: lipid A, core oligosaccharide (OS), and O-specific polysaccharide or O-antigen. Lipid A is the glycolipid endotoxin domain that anchors the LPS molecule to the OM, and therefore, its chemical structure is crucial in the maintenance of membrane integrity in the Gram-negative bacteria. In this paper, we reported the characterization of the lipid A and OS structures from Pseudoalteromonas nigrifaciens Sq02-Rifr, which is a psychrotrophic Gram-negative bacterium isolated from the intestine of Seriola quinqueradiata. The immunomodulatory activity of both LPS and lipid A was also examined.
