Weak Value Amplification Based Optical Sensor for High Throughput Real-Time Immunoassay of SARS-CoV-2 Spike Protein
Xiaonan Zhang,
Lizhong Zhang,
Han Li,
Yang Xu,
Lingqin Meng,
Gengyu Liang,
Bei Wang,
Le Liu,
Tian Guan,
Cuixia Guo,
Yonghong He
Affiliations
Xiaonan Zhang
Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of Optical Imaging and Sensing, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China
Lizhong Zhang
Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of Optical Imaging and Sensing, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China
Han Li
Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of Optical Imaging and Sensing, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China
Yang Xu
Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of Optical Imaging and Sensing, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China
Lingqin Meng
Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of Optical Imaging and Sensing, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China
Gengyu Liang
Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of Optical Imaging and Sensing, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China
Bei Wang
Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of Optical Imaging and Sensing, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China
Le Liu
Institute of Materials Research, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China
Tian Guan
Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of Optical Imaging and Sensing, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China
Cuixia Guo
School of Mechanical Engineering and Automation, Fuzhou University, Fuzhou 350108, China
Yonghong He
Shenzhen Key Laboratory for Minimal Invasive Medical Technologies, Institute of Optical Imaging and Sensing, Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China
The demand for accurate and efficient immunoassays calls for the development of precise, high-throughput analysis methods. This paper introduces a novel approach utilizing a weak measurement interface sensor for immunoassays, offering a solution for high throughput analysis. Weak measurement is a precise quantum measurement method that amplifies the weak value of a system in the weak interaction through appropriate pre- and post-selection states. To facilitate the simultaneous analysis of multiple samples, we have developed a chip with six flow channels capable of conducting six immunoassays concurrently. We can perform real-time immunoassay to determine the binding characteristics of spike protein and antibody through real-time analysis of the flow channel images and calculating the relative intensity. The proposed method boasts a simple structure, eliminating the need for intricate nano processes. The spike protein concentration and relative intensity curve were fitted using the Log-Log fitting regression equation, and R2 was 0.91. Utilizing a pre-transformation approach to account for slight variations in detection sensitivity across different flow channels, the present method achieves an impressive limit of detection(LOD) of 0.85 ng/mL for the SARS-CoV-2 the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein, with a system standard deviation of 5.61. Furthermore, this method has been successfully verified for monitoring molecular-specific binding processes and differentiating binding capacities.
