STAR Protocols (Dec 2021)

Multiplexed imaging and effluent analysis to monitor cancer cell intravasation using a colorectal cancer-on-chip

  • Carly Strelez,
  • Kimya Ghaffarian,
  • Shannon M. Mumenthaler

Journal volume & issue
Vol. 2, no. 4
p. 100984

Abstract

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Summary: Despite colorectal cancer’s (CRC) prevalence, its progression is not well understood. The microfluidic organ-on-chip (OOC) model described herein recreates the epithelial-endothelial tissue-tissue interface, fluid flow, and mechanical forces that exist in vivo, making it an attractive model to understand and ultimately disrupt CRC intravasation. This protocol provides step-by-step details for tumor cell seeding to create a CRC-on-chip model, chip effluent collection and analysis, and on-chip imaging to monitor tumor cell invasion within a more physiologically relevant microenvironment.For complete details on the use and execution of this protocol, please refer to Strelez et al. (2021).