Seasonal variation of behavioural thermoregulation in a fossorial salamander (Ambystoma maculatum)

Abstract

Temperature seasonality plays a pivotal role in shaping the thermal biology of ectotherms. However, we still have a limited understanding of how ectotherms maintain thermal balance in the face of varying temperatures, especially in fossorial species. Due to thermal buffering underground, thermal ecology theory predicts relaxed selection pressure over thermoregulation in fossorial ectotherms. As a result, fossorial ectotherms typically show low thermoregulatory precision and low evidence of thermotactic behaviours in laboratory thermal gradients. Here, we evaluated how temperature selection (Tsel) and associated behaviours differed between seasons in a fossorial amphibian, the spotted salamander (Ambystoma maculatum). By comparing thermoregulatory parameters between the active and overwintering seasons, we show that A. maculatum engages in active behavioural thermoregulation despite being fossorial. In both seasons, Tsel was consistently offset higher than acclimatization temperatures. Thermoregulation differed between seasons, with salamanders having higher Tsel and showing greater evidence of thermophilic behaviours in the active compared with the overwintering season. Additionally, our work lends support to experimental assumptions commonly made but seldom tested in thermal biology studies. Ultimately, our study demonstrates that the combination of careful behavioural and thermal biology measurements is a necessary step to better understand the mechanisms that underlie body temperature control in amphibians.

Keywords

• amphibian
• body temperature
• ectotherm
• fossorial
• seasonality
• thermal biology