Molecular Analysis of mps Operon Involved in Monapterin Syntliesis in Escherichia Coli
Ahn Chiyoung,
Kim Mikyoeng,
Lee Soojin,
Kim Jackwang,
Hwang Deogsu,
Park Kyungmoon,
Yim Jeongbin
Affiliations
Ahn Chiyoung
School of Biological Sciences, Seoul National University, Seoul 151-742, Korea
Kim Mikyoeng
School of Biological Sciences, Seoul National University, Seoul 151-742, Korea
Lee Soojin
Dept. of Microbiology, Chung Nam National University, Dae Jeon 305-764, Korea
Kim Jackwang
School of Biological Sciences, Seoul National University, Seoul 151-742, Korea
Hwang Deogsu
School of Biological Sciences, Seoul National University, Seoul 151-742, Korea
Park Kyungmoon
Dept. of Chemical System Engineering, Hongik University, Yongi-gun, Chung Nam 339-701, Korea
Yim Jeongbin
School of Biological Sciences, College of Natural Sciences, Seoul National University, San 56-1, Shinlim-dong, Kwanak-ku,, Seoul 151-742, Korea, Tel: 82-2-880-6702, Fax: 82-2-871-4315
Two genes, mpsA and mpsB, constitute an operon in the synthesis of monapterin in E. coli. Expression of mpsA, the structural gene for dihydroneopterin triphosphate 2'-epimerase, from its native promoter, required mpsB. Cotransfonnation of mpsB excludes the possibility that the MpsB is a transactivator protein for mpsA expression.
