In Vitro Activity of Sulbactam–Durlobactam against Carbapenem-Resistant <i>Acinetobacter baumannii</i> Clinical Isolates: A Multicentre Report from Italy
Bernardetta Segatore,
Alessandra Piccirilli,
Sabrina Cherubini,
Luigi Principe,
Giovanni Alloggia,
Maria Lina Mezzatesta,
Mario Salmeri,
Stefano Di Bella,
Roberta Migliavacca,
Aurora Piazza,
Elisa Meroni,
Paolo Fazii,
Daniela Visaggio,
Paolo Visca,
Venere Cortazzo,
Giulia De Angelis,
Arianna Pompilio,
Mariagrazia Perilli
Affiliations
Bernardetta Segatore
Department of Biotechnological and Applied Clinical Sciences, University of L’Aquila, 67100 L’Aquila, Italy
Alessandra Piccirilli
Department of Biotechnological and Applied Clinical Sciences, University of L’Aquila, 67100 L’Aquila, Italy
Sabrina Cherubini
Department of Biotechnological and Applied Clinical Sciences, University of L’Aquila, 67100 L’Aquila, Italy
Luigi Principe
Clinical Pathology and Microbiology Unit, “S. Giovanni di Dio” Hospital, 88900 Crotone, Italy
Giovanni Alloggia
Department of Biotechnological and Applied Clinical Sciences, University of L’Aquila, 67100 L’Aquila, Italy
Maria Lina Mezzatesta
Department of Biomedical and Biotechnological Sciences, Section of Microbiology, University of Catania, 95131 Catania, Italy
Mario Salmeri
Department of Biomedical and Biotechnological Sciences, Section of Microbiology, University of Catania, 95131 Catania, Italy
Stefano Di Bella
Clinical Department of Medical, Surgical and Health Sciences, University of Trieste, 34129 Trieste, Italy
Roberta Migliavacca
Unit of Microbiology and Clinical Microbiology, Department of Clinical-Surgical, Diagnostic and Pediatric Sciences, University of Pavia, 27100 Pavia, Italy
Aurora Piazza
Unit of Microbiology and Clinical Microbiology, Department of Clinical-Surgical, Diagnostic and Pediatric Sciences, University of Pavia, 27100 Pavia, Italy
Elisa Meroni
Clinical Microbiology and Virology Unit, “A. Manzoni” Hospital, 23900 Lecco, Italy
Paolo Fazii
Clinical Microbiology and Virology Unit, Spirito Santo Hospital, 65122 Pescara, Italy
Daniela Visaggio
Department of Science, Roma Tre University, Viale G. Marconi 446, 00146 Rome, Italy
Paolo Visca
Department of Science, Roma Tre University, Viale G. Marconi 446, 00146 Rome, Italy
Venere Cortazzo
Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, 00168 Rome, Italy
Giulia De Angelis
Dipartimento di Scienze Biotecnologiche di Base, Cliniche Intensivologiche e Perioperatorie, Università Cattolica del Sacro Cuore, 00168 Rome, Italy
Arianna Pompilio
Department of Medical, Oral and Biotechnological Sciences, “G. d’Annunzio” University of Chieti-Pescara, 66100 Chieti, Italy
Mariagrazia Perilli
Department of Biotechnological and Applied Clinical Sciences, University of L’Aquila, 67100 L’Aquila, Italy
In the present study, the in vitro activity of the sulbactam–durlobactam (SUL–DUR) combination was evaluated against 141 carbapenem-resistant A. baumannii (CRAb) clinical strains collected from six Italian laboratories. Over half (54.6%) of these isolates were resistant to colistin. The SUL–DUR combination was active against these CRAb isolates with MIC50 and MIC90 values of 0.5 mg/L and 4 mg/L, respectively. Only eleven isolates were resistant to SUL–DUR with MIC values ranging from 8 to 128 mg/L. The SUL–DUR resistant A. baumannii exhibited several antimicrobial resistance genes (ARGs) such as blaOXA-20, blaOXA-58, blaOXA-66, blaADC-25, aac(6′)-Ib3 and aac(6′)-Ib-cr and mutations in gyrA (S81L) and parC (V104I, D105E). However, in these isolates, mutations Q488K and Y528H were found in PBP3. Different determinants were also identified in these CRAb isolates, including adeABC, adeFGH, adeIJK, abeS, abaQ and abaR, which encode multidrug efflux pumps associated with resistance to multiple antibacterial agents. This is the first report on the antimicrobial activity of SUL–DUR against carbapenem-resistant A. baumannii isolates selected from multiple regions in Italy.
