Inference and effects of barcode multiplets in droplet-based single-cell assays

Caleb A. Lareau; Sai Ma; Fabiana M. Duarte; Jason D. Buenrostro

doi:10.1038/s41467-020-14667-5

Nature Communications (Feb 2020)

Inference and effects of barcode multiplets in droplet-based single-cell assays

Caleb A. Lareau,
Sai Ma,
Fabiana M. Duarte,
Jason D. Buenrostro

Affiliations

Caleb A. Lareau: Department of Stem Cell and Regenerative Biology, Harvard University
Sai Ma: Department of Stem Cell and Regenerative Biology, Harvard University
Fabiana M. Duarte: Department of Stem Cell and Regenerative Biology, Harvard University
Jason D. Buenrostro: Department of Stem Cell and Regenerative Biology, Harvard University

DOI: https://doi.org/10.1038/s41467-020-14667-5
Journal volume & issue: Vol. 11, no. 1
pp. 1 – 9

Abstract

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It is assumed that single-cell analyses capture one barcode per cell. Here, the authors show that up to 21% of cell barcodes on the 10X Chromium scATAC-seq assay may be derived from barcode multiplets.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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