Exercise training restores IGFIR survival signaling in d-galactose induced-aging rats to suppress cardiac apoptosis

Ing-Shiow Lay; Wei-Wen Kuo; Marthandam Asokan Shibu; Tsung-Jung Ho; Shiu-Min Cheng; Cecilia Hsuan Day; Bo Ban; Shulin Wang; Qiaowen Li; Chih-Yang Huang

Journal of Advanced Research (Feb 2021)

Exercise training restores IGFIR survival signaling in d-galactose induced-aging rats to suppress cardiac apoptosis

Ing-Shiow Lay,
Wei-Wen Kuo,
Marthandam Asokan Shibu,
Tsung-Jung Ho,
Shiu-Min Cheng,
Cecilia Hsuan Day,
Bo Ban,
Shulin Wang,
Qiaowen Li,
Chih-Yang Huang

Affiliations

Ing-Shiow Lay: School of Chinese Medicine, College of Chinese Medicine, China Medical University, 40402 Taichung, Taiwan; Department of Chinese Medicine, China Medical University Beigang Hospital, Yunlin County 65152, Taiwan
Wei-Wen Kuo: Department of Biological Science and Technology, China Medical University, Taichung 40402, Taiwan
Marthandam Asokan Shibu: Cardiovascular and Mitochondrial Related Disease Research Center, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien 970, Taiwan
Tsung-Jung Ho: Integration Center of Traditional Chinese and Modern Medicine, HualienTzu Chi Hospital, Hualien 97002, Taiwan; Department of Chinese Medicine,Hualien Tzu Chi Hospital, Hualien 97002, Taiwan; School of Post Baccalaureate Chinese Medicine, College of Medicine, Tzu Chi University, Hualien 97004, Taiwan
Shiu-Min Cheng: Department of Psychology, Asia University, Taichung, Taiwan
Cecilia Hsuan Day: Department of Nursing, Meiho University, Pingtung, Taiwan
Bo Ban: Department of Endocrinology, Affiliated Hospital of Jining Medical University, Jining Medical University, 89 Guhuai Road, Jining, Shandong 272029, China
Shulin Wang: Department of Cardiology, The Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People’s Hospital, Qingyuan 511518, Guangdong, China
Qiaowen Li: Department of Cardiology, The Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan People’s Hospital, Qingyuan 511518, Guangdong, China
Chih-Yang Huang: School of Chinese Medicine, College of Chinese Medicine, China Medical University, 40402 Taichung, Taiwan; Cardiovascular and Mitochondrial Related Disease Research Center, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien 970, Taiwan; Department of Biotechnology, Asia University, Taichung, Taiwan; Department of Medical Research, China Medical University Hospital, Taichung, Taiwan; Holistic Education Center, Tzu Chi University of Science and Technology, Hualien, Taiwan; Corresponding author at: Cardiovascular and Mitochondrial Related Disease Research Center, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.

Journal volume & issue: Vol. 28
pp. 35 – 41

Abstract

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Introduction: Insulin-like growth factor-I receptor (IGF1R) mediated survival signaling is a crucial mechanism for cellular endurance and a potential indicator of recuperation in deteriorating hearts. Objective: This study evaluates the impact of long-term exercise training in enhancing cardiac survival mechanism in D-galactose-induced toxicity associated aging rats. Methods: Forty-eight male SD-rats were segregated into 4 groups (n=9) and were named as control, exercise training groups, aging group and aging group with exercise training. Aging was induced by intraperitoneal (IP) D-galactose (150 mL/kg) injection for 8 weeks and for exercise training, the rats were left to swim in warm water for 60 min every day and 5 times/week. Western blotting of proteins from the left ventricles was performed to identify the modulations in the survival signaling. Tissue sections were analyzed to determine the extent of fibrosis and apoptosis. Results: Western-blot analysis performed on the excised left ventricles (LV) showed that proteins of the cardiac survival pathway including IGF1R and Akt and the pro-survival Bcl-2 showed significant decrease in the aging group, whereas the levels were restored in the aging rats subjected to exercise training. In addition, aging groups showed increased interstitial space and collagen accumulation. Further, TUNEL assay showed higher number of apoptotic cells in the LV of aging group, which was correlated with increase in the proteins involved in FAS-FADD-dependent apoptosis. However, these aging associated effects were ameliorated upon exercise training in the D-galactose-induced aging rats that showed elevated IGF1R/Akt signaling. Conclusion: The results suggest that IGFIR survival signaling cascadeis elevated in following long-term exercise training and thereby provide cardio-protective benefits in D-galactose induced aging rats.

Published in Journal of Advanced Research

ISSN: 2090-1232 (Print); 2090-1224 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Medicine: Medicine (General); Science: Science (General)
Website: http://www.journals.elsevier.com/journal-of-advanced-research/

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