Escherichia coli Penghasil Extended-spectrum Β-Lactamases yang Diisolasi pada Peternakan Broiler-Ikan Terintegrasi

Abstract

Extended-spectrum β-lactamases (ESBL) are a group of enzymes producing bacteria that lead to antibiotic resistance, including to the third generation of cephalosporin, and become a major health concern in humans and animals. This study aimed to determine the presence of ESBL-producing Escherichia coli and their resistance profile in environmental components around an integrated broiler-fish farming. ESBL-producing E. coli and antibiotic susceptibility were tested using double disc synergy and disc diffusion tests, respectively, and the results were interpreted based on The European Committee on Antimicrobial Susceptibility Testing guideline. 19 ESBL-producing E. coli were isolated from 21 cloacal and environmental swab samples from an integrated broiler-fish farm. ESBL-producing E. coli were detected from cloacal swab samples (13/30; 86.6%), water samples (3/3; 100%), fish samples (2/2; 100%), and soil sample (1/1; 100%). The antibiotic resistance test showed resistance to ampicillin (100%), ceftriaxone (100%), ciprofloxacin (69.2%), tetracycline (46.15%), and colistin (0%). In addition, eight isolates consisting of swab samples (6/13; 46.1%), water (1/3; 33.3%), and fish (1/2; 50.0%) were identified as multi-drug resistant bacteria. The presence of ESBL-producing E. coli isolated from an integrated broiler-fish farming could describe the occurrence of transmission-resistant bacteria in the environment.

Keywords

• escherichia coli
• extended-spectrum β-lactamases
• broiler
• fish
• environment