Validation of the Method for Dosing Linoleic Acid in Food Animal Products

Abstract

One of the big problems of chemical analysis is to ensure its quality and to increase the confidence in the analytical results yielded by a chemical or instrumental analysis. The new internal norms for animal products quality (ANSVSA order 51/2005 regarding the performance of the analytical methods of analysis and data interpretation), aligned to EU requirements, required a unique system of inspection. These requirements stipulate the obligation of the laboratories inspecting animal products to validate each assay method, adapted to the particular type of sample. We therefore aimed to validate the chromatographic method of determining the linoleic acid from meat products. The parameters determined for the validation of this method were in agreement with SR EN ISO/CEI 17025:2005 and refer to: accuracy, reproducibility, sensitivity, precision, limit of detection, and limit of quantification. For validation we used only reference materials and certified standards of analytical purity. The recovery was 95.60% and the incertitude was±0.69 for a sample of 20.673% linoleic acid concentration of total fatty acid. The results prove that the method operates within the admitted limits and is adequate for linoleic acid determination in animal products.

Keywords

• animal products
• chromatography
• linoleic acid
• method validation
• quality