Karyotypic Study of Taylor’s Stream Frog (Limnonectes taylori) in Chiang Mai, Thailand

Abstract

This study analyzed the karyotype and idiogram of Taylor’s stream frog (Limnonectes taylori) collected from Chiang Mai Province in Northern Thailand. A mitotic chromosome preparation from bone marrow was obtained by direct method. Chromosomes were stained by conventional staining technique with 20% Giemsa solution in order to examine diploid number, shape and size. The result showed that the diploid number (2n) of Limnonectes taylori was 22, the fundamental number (NF) was 44 both male and female. The type of chromosomes were 8 large metacentrics, 2 large submetacentrics, 2 medium metacentrics, 2 medium submetacentrics and 8 small metacentrics. No cytologically distinguishable sex chromosome was observed. The karyotype formula of L. taylori is as follows: 2n (22) = Lm 8 + Lsm 2 + Mm 2 + Msm 2 + Sm 8

Keywords

• chromosome
• karyotype
• limnonectes taylori