Obtaining the tbf gene which encodes immunodominant epitopes of pathogenic cholera strains

Abstract

We experimentally carried out the synthesis of the tbf gene that encodes highly-immunogenic epitopes of pathogenic cholera strains, through the cloning of the tbf gene in pGEM-T Easy plasmid. Moreover, we tested the hybrid gene sequence for absence of mutations, using the Sanger sequencing. We also calculated the nucleatic sequence of the tbf gene. The obtained results have both scientific and practical significance.

Keywords

• candidate cholera vaccine
• hybrid tbf gene
• sanger sequencing
• protein design
• tbf recombinant protein
• immunodominant epitopes
• vibrio choleraе