OncoTargets and Therapy (Aug 2020)

Long Noncoding RNA HIF1A-AS2 Promotes Non-Small Cell Lung Cancer Progression by the miR-153-5p/S100A14 Axis

  • Zhang W,
  • Liu K,
  • Pei Y,
  • Tan J,
  • Ma J,
  • Zhao J

Journal volume & issue
Vol. Volume 13
pp. 8715 – 8722

Abstract

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Weiqiang Zhang, Keqiang Liu, Yingxin Pei, Jian Tan, Jingbo Ma, Jing Zhao Department of Thoracic Surgery, The 7th Medical Center of PLA General Hospital, Beijing 100700, People’s Republic of ChinaCorrespondence: Jing ZhaoDepartment of Thoracic Surgery, The 7th Medical Center of PLA General Hospital, No. 5, Nanmencang Street, Dongcheng District, Beijing 100700, People’s Republic of ChinaEmail [email protected]: Long noncoding RNA (lncRNA) plays a critical role in initiating lung cancer. This study aims to research the function and mechanism of lncRNA HIF1A-AS2 in regulating non-small cell lung cancer (NSCLC) progression.Methods: qRT-PCR was used to analyze gene expression. The CCK-8 assay was performed to detect cell proliferation. The Transwell assay was conducted to examine cell migration and invasion. A Caspase3 activity detection kit was utilized to analyze apoptosis. The luciferase reporter assay was carried out to research interactions of HIF1A-AS2, miR-153-5p and S100A14.Results: HIF1A-AS2 expression was raised in NSCLC tissues and cell lines. The HIF1A-AS2 level was increased in advanced NSCLC tumor tissues. High HIF1A-AS2 expression was related to poor prognosis. HIF1A-AS2 knockdown decreased proliferation, migration and invasion while promoting apoptosis. HIF1A-AS2 was the sponge for miR-153-5p, and miR-153-5p targeted S100A14. HIF1A-AS2 promoted S100A14 expression through regulating miR-153-5p.Conclusion: The HIF1A-AS2/miR-153-5p/S100A14 axis plays a crucial role in promoting NSCLC progression.Keywords: NSCLC, HIF1A-AS2, miR-153-5p, S100A14

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