International Journal of Nanomedicine (Sep 2018)
Antibacterial and biodegradable tissue nano-adhesives for rapid wound closure
Abstract
Meng-meng Lu,1,2 Jing Bai,3 Dan Shao,4,5 Jing Qiu,1,2 Ming Li,1,2 Xiao Zheng,5 Yun Xiao,6 Zheng Wang,4 Zhi-min Chang,4 Li Chen,5 Wen-fei Dong,4 Chun-bo Tang1,2 1Department of Oral Implantology, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing, 210029, China; 2Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing, 210029, China; 3School of Materials Science and Engineering, Southeast University, Jiangsu Key Laboratory for Advanced Metallic Materials, Jiangning, Nanjing 211189, Jiangsu, China; 4CAS Key Laboratory of Bio Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China; 5Department of Pharmacology, College of Basic Medical Sciences, School of Nursing, Jilin University, Changchun 130021, China; 6National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610064, China Background: Although various organic tissue adhesives designed to facilitate would healing are gaining popularity in diverse clinical applications, they present significant inherent limitations, such as rejection, infections, toxicity and/or excessive swelling. It is highly desirable to develop efficient, biocompatible and anti-bacterial tissue adhesives for skin wound healing. Purpose: Inspired by the fact that inorganic nanoparticles can directly glue tissues through the “nanobridging effect”, herein disulfide bond-bridged nanosilver-decorated mesoporous silica nanoparticles (Ag-MSNs) was constructed as an effective and safe tissue adhesive with antibacterial and degradable properties for wound closure and healing. Materials and methods: Ag-MSNs was fabricated by controlled reduce of ultrasmall nanosilvers onto the both surface and large pore of biodegradable MSNs. The obtained MSNs were characterized by transmission electron microscopy, Fourier transform infrared spectroscopy, energy-dispersive X-ray spectroscopy, and measurement of size distribution, zeta potential, and mesopore properties. Furthermore, adhesion strength test, anti-bacterial assessment, mouse skin wound model, and MTT assays were used to investigate the tissue adhesive property, anti-bacterial effect, biodegradability and biocompatibility of the Ag-MSNs. Results: Ag-MSNs exhibited not only strong adhesive properties but also excellent antibacterial activities than that of MSNs. Importantly, this antibacterial nano-adhesive achieved rapid and efficient closure and healing of wounds in comparison to sutures or MSNs in a mouse skin wound model. Furthermore, Ag-MSNs with fast degradable behavior caused little cellular toxicity and even less systemic toxicity during wound healing. Conclusion: Our findings suggest that biodegradable Ag-MSNs can be employed as the next generation of nano-adhesives for rapid wound closure and aesthetic wound healing. Keywords: nanosilver-decorated mesoporous silica nanoparticles, nano-adhesive, wound healing, antibacterial, biodegradable
