Production and Characterization of Active Bacterial Cellulose Films Obtained from the Fermentation of Wine Bagasse and Discarded Potatoes by <i>Komagateibacter xylinus</i>

Abstract

Potato waste, such as peels, broken or spoiled potatoes and grape bagasse residues from the winery industry, can be used for the biotechnological production of high-value products. In this study, green, sustainable and highly productive technology was developed for the production of antioxidant bacterial cellulose (BC). The aim of this work was to evaluate the feasibility of a low-cost culture medium based on wine bagasse and potato waste to synthesize BC. Results show that the production of BC by Komagateibacter xylinus in the GP culture medium was five-fold higher than that in the control culture medium, reaching 4.0 g/L BC in 6 days. The compounds of the GP culture medium improved BC production yield. The mechanical, permeability, swelling capacity, antioxidant capacity and optical properties of the BC films from the GP medium were determined. The values obtained for the tensile and puncture properties were 22.77 MPa for tensile strength, 1.65% for elongation at break, 910.46 MPa for Young’s modulus, 159.31 g for burst strength and 0.70 mm for distance to burst. The obtained films showed lower permeability values (3.40 × 10−12 g/m·s·Pa) than those of other polysaccharide-based films. The BC samples showed an outstanding antioxidant capacity (0.31–1.32 mg GAE/g dried film for total phenolic content, %DPPH• 57.24–78.00% and %ABTS•+ 89.49–86.94%) and excellent UV-barrier capacity with a transmittance range of 0.02–0.38%. Therefore, a new process for the production of BC films with antioxidant properties was successfully developed.

Keywords

• bacterial cellulose
• agricultural waste
• <i>Komagateibacter xylinus</i>
• active film
• antioxidant properties
• ABTS