Isolation and characterization of a novel transcriptional repressor GmERF6 from soybean

Y. Zhai; J. -W. Li; X. -W. Li; T. -T. Lei; F. Yan; Y. Zhao; Y. -J. Li; L. -T. Su; Y. Wang; Q. -Y. Wang

doi:10.1007/s10535-012-0146-7

Biologia Plantarum (Mar 2013)

Isolation and characterization of a novel transcriptional repressor GmERF6 from soybean

Y. Zhai,
J. -W. Li,
X. -W. Li,
T. -T. Lei,
F. Yan,
Y. Zhao,
Y. -J. Li,
L. -T. Su,
Y. Wang,
Q. -Y. Wang

Affiliations

Y. Zhai: College of Plant Science, Jilin University, Changchun, Jilin, P.R. China
J. -W. Li: College of Plant Science, Jilin University, Changchun, Jilin, P.R. China
X. -W. Li: Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, Jilin, P.R. China
T. -T. Lei: College of Plant Science, Jilin University, Changchun, Jilin, P.R. China
F. Yan: College of Plant Science, Jilin University, Changchun, Jilin, P.R. China
Y. Zhao: College of Life Science and Agroforestry, Qiqihaer University, Qiqihaer, Heilongjiang, P.R. China
Y. -J. Li: College of Plant Science, Jilin University, Changchun, Jilin, P.R. China
L. -T. Su: College of Plant Science, Jilin University, Changchun, Jilin, P.R. China
Y. Wang: College of Plant Science, Jilin University, Changchun, Jilin, P.R. China
Q. -Y. Wang: College of Plant Science, Jilin University, Changchun, Jilin, P.R. China

DOI: https://doi.org/10.1007/s10535-012-0146-7
Journal volume & issue: Vol. 57, no. 1
pp. 26 – 32

Abstract

Read online

A new ethylene response factor (ERF), GmERF6, was isolated from soybean. Protein sequence alignment of GmERF6 revealed an AP2/ERF domain, two putative nuclear localization signals (NLSs) and an ERF-associated amphiphilic repression (EAR) motif. Real-time quantitative PCR analysis revealed that the expression of GmERF6 was differentially induced in soybean seedlings by drought, salt, cold, salicylic acid, ethylene, abscisic acid and methyl jasmonate. Transient expression experiments demonstrated that GmERF6 functions as a transcriptional repressor to downregulate the transcriptional levels of the reporter gene and repress the activated ability of other transcriptional activator. Transgenic Arabidopsis lines constitutively expressing GmERF6 showed an increased tolerance to drought compared to wild-type plants.

Published in Biologia Plantarum

ISSN: 1573-8264 (Online)
Publisher: Institute of Experimental Botany of the Czech Academy of Sciences
Country of publisher: Czechia
LCC subjects: Science: Biology (General); Science: Botany: Plant ecology
Website: https://bp.ueb.cas.cz/

About the journal

Abstract

Keywords