Isolation of spermatogenic cells from the cynomolgus macaque testis with flow cytometry

Abstract

Summary: Here, we describe a detailed protocol for the isolation of purified populations of viable spermatogenic cells derived from the non-human primate model organism Macaca fascicularis (cynomolgus). Using fluorescence-activated cell sorting (FACS), we describe methods to isolate spermatogonia and primary spermatocytes ranging across the sub-stages of meiosis prophase I. These cell populations can be used with a variety of downstream assays, including single-cell approaches such as RNA sequencing, chromatin immunoprecipitation, quantitative RT-PCR, and immunocytochemistry.For complete details on the use and execution of this protocol, please refer to Lau et al. (2020).

Keywords

• Cell biology
• Cell isolation
• Single cell
• Flow cytometry/mass cytometry
• Model organisms