Nature Communications (Jul 2022)
De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals
- Guillaume Tetreau,
- Michael R. Sawaya,
- Elke De Zitter,
- Elena A. Andreeva,
- Anne-Sophie Banneville,
- Natalie A. Schibrowsky,
- Nicolas Coquelle,
- Aaron S. Brewster,
- Marie Luise Grünbein,
- Gabriela Nass Kovacs,
- Mark S. Hunter,
- Marco Kloos,
- Raymond G. Sierra,
- Giorgio Schiro,
- Pei Qiao,
- Myriam Stricker,
- Dennis Bideshi,
- Iris D. Young,
- Ninon Zala,
- Sylvain Engilberge,
- Alexander Gorel,
- Luca Signor,
- Jean-Marie Teulon,
- Mario Hilpert,
- Lutz Foucar,
- Johan Bielecki,
- Richard Bean,
- Raphael de Wijn,
- Tokushi Sato,
- Henry Kirkwood,
- Romain Letrun,
- Alexander Batyuk,
- Irina Snigireva,
- Daphna Fenel,
- Robin Schubert,
- Ethan J. Canfield,
- Mario M. Alba,
- Frédéric Laporte,
- Laurence Després,
- Maria Bacia,
- Amandine Roux,
- Christian Chapelle,
- François Riobé,
- Olivier Maury,
- Wai Li Ling,
- Sébastien Boutet,
- Adrian Mancuso,
- Irina Gutsche,
- Eric Girard,
- Thomas R. M. Barends,
- Jean-Luc Pellequer,
- Hyun-Woo Park,
- Arthur D. Laganowsky,
- Jose Rodriguez,
- Manfred Burghammer,
- Robert L. Shoeman,
- R. Bruce Doak,
- Martin Weik,
- Nicholas K. Sauter,
- Brian Federici,
- Duilio Cascio,
- Ilme Schlichting,
- Jacques-Philippe Colletier
Affiliations
- Guillaume Tetreau
- Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
- Michael R. Sawaya
- UCLA-DOE Institute for Genomics and Proteomics, Department of Biological Chemistry, University of California
- Elke De Zitter
- Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
- Elena A. Andreeva
- Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
- Anne-Sophie Banneville
- Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
- Natalie A. Schibrowsky
- UCLA-DOE Institute for Genomics and Proteomics, Department of Biological Chemistry, University of California
- Nicolas Coquelle
- Large-Scale Structures Group, Institut Laue-Langevin
- Aaron S. Brewster
- Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory
- Marie Luise Grünbein
- Max-Planck-Institut für medizinische Forschung
- Gabriela Nass Kovacs
- Max-Planck-Institut für medizinische Forschung
- Mark S. Hunter
- Linac Coherent Light Source, SLAC National Accelerator Laboratory
- Marco Kloos
- Max-Planck-Institut für medizinische Forschung
- Raymond G. Sierra
- Linac Coherent Light Source, SLAC National Accelerator Laboratory
- Giorgio Schiro
- Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
- Pei Qiao
- Department of Chemistry, Texas A&M University
- Myriam Stricker
- Max-Planck-Institut für medizinische Forschung
- Dennis Bideshi
- Department of Entomology and Institute for Integrative Genome Biology, University of California
- Iris D. Young
- Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory
- Ninon Zala
- Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
- Sylvain Engilberge
- Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
- Alexander Gorel
- Max-Planck-Institut für medizinische Forschung
- Luca Signor
- Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
- Jean-Marie Teulon
- Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
- Mario Hilpert
- Max-Planck-Institut für medizinische Forschung
- Lutz Foucar
- Max-Planck-Institut für medizinische Forschung
- Johan Bielecki
- European XFEL GmbH
- Richard Bean
- European XFEL GmbH
- Raphael de Wijn
- European XFEL GmbH
- Tokushi Sato
- European XFEL GmbH
- Henry Kirkwood
- European XFEL GmbH
- Romain Letrun
- European XFEL GmbH
- Alexander Batyuk
- Linac Coherent Light Source, SLAC National Accelerator Laboratory
- Irina Snigireva
- European Synchrotron Radiation Facility (ESRF), BP 220
- Daphna Fenel
- Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
- Robin Schubert
- European XFEL GmbH
- Ethan J. Canfield
- Mass Spectrometry Core Facility, School of Pharmacy, University of Southern California
- Mario M. Alba
- Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California
- Frédéric Laporte
- Univ. Grenoble Alpes, CNRS, LECA
- Laurence Després
- Univ. Grenoble Alpes, CNRS, LECA
- Maria Bacia
- Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
- Amandine Roux
- Univ. Lyon, ENS de Lyon, CNRS UMR 5182, Université Claude Bernard Lyon 1, Laboratoire de Chimie
- Christian Chapelle
- Polyvalan SARL
- François Riobé
- Univ. Lyon, ENS de Lyon, CNRS UMR 5182, Université Claude Bernard Lyon 1, Laboratoire de Chimie
- Olivier Maury
- Univ. Lyon, ENS de Lyon, CNRS UMR 5182, Université Claude Bernard Lyon 1, Laboratoire de Chimie
- Wai Li Ling
- Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
- Sébastien Boutet
- Linac Coherent Light Source, SLAC National Accelerator Laboratory
- Adrian Mancuso
- European XFEL GmbH
- Irina Gutsche
- Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
- Eric Girard
- Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
- Thomas R. M. Barends
- Max-Planck-Institut für medizinische Forschung
- Jean-Luc Pellequer
- Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
- Hyun-Woo Park
- Department of Entomology and Institute for Integrative Genome Biology, University of California
- Arthur D. Laganowsky
- Department of Chemistry, Texas A&M University
- Jose Rodriguez
- UCLA-DOE Institute for Genomics and Proteomics, Department of Biological Chemistry, University of California
- Manfred Burghammer
- European Synchrotron Radiation Facility (ESRF), BP 220
- Robert L. Shoeman
- Max-Planck-Institut für medizinische Forschung
- R. Bruce Doak
- Max-Planck-Institut für medizinische Forschung
- Martin Weik
- Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
- Nicholas K. Sauter
- Molecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory
- Brian Federici
- Department of Entomology and Institute for Integrative Genome Biology, University of California
- Duilio Cascio
- UCLA-DOE Institute for Genomics and Proteomics, Department of Biological Chemistry, University of California
- Ilme Schlichting
- Max-Planck-Institut für medizinische Forschung
- Jacques-Philippe Colletier
- Univ. Grenoble Alpes, CNRS, CEA, Institut de Biologie Structurale
- DOI
- https://doi.org/10.1038/s41467-022-31746-x
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 18
Abstract
An environmentally safe means of mosquito control is the application of Bacillus thuringiensis israelensis, which produces a cocktail of four naturally crystalline proteins exclusively toxic to mosquito. Here the authors report the atomic-resolution structures of Bti Cry11Aa and related Btj Cry11Ba solved de novo through Serial Femtosecond Crystallography on naturally-occurring nanocrystals.
