Surgical protocol for permanent ligation of the left anterior descending coronary artery in mice to generate a model of myocardial infarction

Kazuaki Maruyama; Kazuaki Naemura; Kenji Yoshihara; Kyoko Imanaka-Yoshida; Hiroki Kurihara; Sachiko Miyagawa-Tomita

STAR Protocols (Sep 2021)

Surgical protocol for permanent ligation of the left anterior descending coronary artery in mice to generate a model of myocardial infarction

Kazuaki Maruyama,
Kazuaki Naemura,
Kenji Yoshihara,
Kyoko Imanaka-Yoshida,
Hiroki Kurihara,
Sachiko Miyagawa-Tomita

Affiliations

Kazuaki Maruyama: Department of Physiological Chemistry and Metabolism, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan; Department of Pathology and Matrix Biology, Graduate School of Medicine, Mie University Graduate School of Medicine, 2-174 Edobashi Tsu, Mie 514-8507, Japan; Corresponding author
Kazuaki Naemura: Department of Physiological Chemistry and Metabolism, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
Kenji Yoshihara: Heart Center, Department of Pediatric Cardiology, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan
Kyoko Imanaka-Yoshida: Department of Pathology and Matrix Biology, Graduate School of Medicine, Mie University Graduate School of Medicine, 2-174 Edobashi Tsu, Mie 514-8507, Japan
Hiroki Kurihara: Department of Physiological Chemistry and Metabolism, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan
Sachiko Miyagawa-Tomita: Department of Physiological Chemistry and Metabolism, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan; Heart Center, Department of Pediatric Cardiology, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan; Department of Animal Nursing Science, Yamazaki University of Animal Health Technology, 4-7-2 Minami-Osawa, Hachioji, Tokyo 192-0364, Japan; Corresponding author

Journal volume & issue: Vol. 2, no. 3
p. 100775

Abstract

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Summary: Myocardial infarction (MI) is one of the most common causes of death worldwide. Animal models for MI are useful for studying the pathophysiology and developing therapies. Here, we describe a surgical protocol for permanent ligation of the left anterior descending coronary artery in mice, which mimics human acute coronary syndrome. This protocol includes descriptive step-by-step surgical procedures and high-quality surgical videos, which are useful for performing stable and highly reproducible operations.For complete details on the use and execution of this protocol, please refer to Maruyama et al. (2021)

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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