Biotecnología en el Sector Agropecuario y Agroindustrial (Dec 2008)
EVALUACIÓN DEL EFECTO DE DIFERENTES CONCENTRACIONES DE FITOHORMONAS EN LA MICROPROPAGACIÓN DEL PLÁTANO DOMINICO HARTÓN (Musa AAB Simmonds) EVALUATION OF THE EFFECT OF DIFFERENT CONCENTRATIONS OF PHYTOHORMONES IN MICROPROPAGATION OF DOMINICO HARTÓN PLANTAIN (Musa AAB Simmonds)
Abstract
Se evaluó el efecto de la concentración hormonal en la generación de brotes al micropropagar plátano dominico hartón (Musa AAB Simmonds) con 10 tratamientos en un diseño completamente al azar (DCA). Se probaron tres concentraciones de BAP y AIA en arreglo factorial 3x3: 0.01; 0.5 y 5 mg/L. Los tratamientos presentaron diferencias significativas (pThe effect of the hormonal concentration in the generation of bud in micropropagation of dominico-harton plantain (Musa AAB Simmonds) was evaluated with 10 treatments in a Complete Randomized Design (CRD). Three concentrations of BAP and IAA in a 3x3 factorial arrangement: 0,01; 0,5 and 5 mg/L were tested. The treatments presented significant differences (p< 0.01%) in vitro multiplication phase. The MSDt test showed that the major number of bud were found with 5mg/L of BAP / 0.5mg/L of AIA and 5mg/L of BAP / 0.01mg/L of AIA combinations. To determine the optimum concentration of AIA from the resultant rank (0,01-0,5 mg/L), an aditional experiment with 6 treatments in a Complete Randomized Design (CRD), varying only AIA concentration was conducted. The concentrations 0.2 and 0,3 mg/L of AIA (p< 0.01%) generated the biggest number of sprouts in the stage of multiplication