CD83 expression induced by CpG-DNA stimulation in a macrophage cell line RAW 264.7

Abstract

CpG-DNA has various immunomodulatory effects in dendriticcells, B cells, and macrophages. While induction of cytokinesby CpG-DNA has been well documented in macrophages, theexpression of costimulatory molecules in CpG-DNA treatedmacrophages has not yet been defined. Therefore, weinvestigated the effects of CpG-DNA on the expression ofcostimulatory molecules in RAW 264.7 cells. The surfaceexpression of CD80 was slightly increased and CD83expression was significantly increased in response toCpG-DNA. However, the expression of CD86 and MHC classII was not changed. As expression of CD83 mRNA was alsoincreased by CpG-DNA, CD83 expression is regulated at atranscriptional level. To understand the contribution ofsignaling pathways to CD83 induction, we used pathwayspecific inhibitors. The NF-κB inhibitor significantly reducedsurface expression of CD83 as well as phagocytic activity ofRAW 264.7 cells. Therefore, CD83 expression may contributeto the immunostimulatory effects of CpG-DNA in macrophagecells. [BMB Reports 2013; 46(9): 448-453]

Keywords

• CD83
• Costimulatory molecule
• CpG-DNA
• Innate immunity
• Macrophage