Micromachines (Apr 2024)

Extending the Depth of Focus of an Infrared Microscope Using a Binary Axicon Fabricated on Barium Fluoride

  • Molong Han,
  • Daniel Smith,
  • Tauno Kahro,
  • Dominyka Stonytė,
  • Aarne Kasikov,
  • Darius Gailevičius,
  • Vipin Tiwari,
  • Agnes Pristy Ignatius Xavier,
  • Shivasubramanian Gopinath,
  • Soon Hock Ng,
  • Aravind Simon John Francis Rajeswary,
  • Aile Tamm,
  • Kaupo Kukli,
  • Keith Bambery,
  • Jitraporn Vongsvivut,
  • Saulius Juodkazis,
  • Vijayakumar Anand

DOI
https://doi.org/10.3390/mi15040537
Journal volume & issue
Vol. 15, no. 4
p. 537

Abstract

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Axial resolution is one of the most important characteristics of a microscope. In all microscopes, a high axial resolution is desired in order to discriminate information efficiently along the longitudinal direction. However, when studying thick samples that do not contain laterally overlapping information, a low axial resolution is desirable, as information from multiple planes can be recorded simultaneously from a single camera shot instead of plane-by-plane mechanical refocusing. In this study, we increased the focal depth of an infrared microscope non-invasively by introducing a binary axicon fabricated on a barium fluoride substrate close to the sample. Preliminary results of imaging the thick and sparse silk fibers showed an improved focal depth with a slight decrease in lateral resolution and an increase in background noise.

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