A Resource for Manipulating Gene Expression and Analyzing cis-Regulatory Modules in the Drosophila CNS
Laurina Manning,
Ellie S. Heckscher,
Maria D. Purice,
Jourdain Roberts,
Alysha L. Bennett,
Jason R. Kroll,
Jill L. Pollard,
Marie E. Strader,
Josh R. Lupton,
Anna V. Dyukareva,
Phuong Nam Doan,
David M. Bauer,
Allison N. Wilbur,
Stephanie Tanner,
Jimmy J. Kelly,
Sen-Lin Lai,
Khoa D. Tran,
Minoree Kohwi,
Todd R. Laverty,
Joseph C. Pearson,
Stephen T. Crews,
Gerald M. Rubin,
Chris Q. Doe
Affiliations
Laurina Manning
Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, OR 97403, USA
Ellie S. Heckscher
Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, OR 97403, USA
Maria D. Purice
Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, OR 97403, USA
Jourdain Roberts
Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, OR 97403, USA
Alysha L. Bennett
Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, OR 97403, USA
Jason R. Kroll
Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, OR 97403, USA
Jill L. Pollard
Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, OR 97403, USA
Marie E. Strader
Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, OR 97403, USA
Josh R. Lupton
Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, OR 97403, USA
Anna V. Dyukareva
Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, OR 97403, USA
Phuong Nam Doan
Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, OR 97403, USA
David M. Bauer
Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, OR 97403, USA
Allison N. Wilbur
Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, OR 97403, USA
Stephanie Tanner
Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, OR 97403, USA
Jimmy J. Kelly
Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, OR 97403, USA
Sen-Lin Lai
Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, OR 97403, USA
Khoa D. Tran
Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, OR 97403, USA
Minoree Kohwi
Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, OR 97403, USA
Todd R. Laverty
Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
Joseph C. Pearson
Program in Molecular Biology and Biophysics, Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
Stephen T. Crews
Program in Molecular Biology and Biophysics, Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA
Gerald M. Rubin
Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA
Chris Q. Doe
Institutes of Neuroscience and Molecular Biology, Howard Hughes Medical Institute, University of Oregon, Eugene, OR 97403, USA
Here, we describe the embryonic central nervous system expression of 5,000 GAL4 lines made using molecularly defined cis-regulatory DNA inserted into a single attP genomic location. We document and annotate the patterns in early embryos when neurogenesis is at its peak, and in older embryos where there is maximal neuronal diversity and the first neural circuits are established. We note expression in other tissues, such as the lateral body wall (muscle, sensory neurons, and trachea) and viscera. Companion papers report on the adult brain and larval imaginal discs, and the integrated data sets are available online (http://www.janelia.org/gal4-gen1). This collection of embryonically expressed GAL4 lines will be valuable for determining neuronal morphology and function. The 1,862 lines expressed in small subsets of neurons (<20/segment) will be especially valuable for characterizing interneuronal diversity and function, because although interneurons comprise the majority of all central nervous system neurons, their gene expression profile and function remain virtually unexplored.
