Frontiers in Plant Science (Jun 2022)
Gene Co-expression Network Analysis of the Comparative Transcriptome Identifies Hub Genes Associated With Resistance to Aspergillus flavus L. in Cultivated Peanut (Arachis hypogaea L.)
- Mengjie Cui,
- Mengjie Cui,
- Mengjie Cui,
- Mengjie Cui,
- Mengjie Cui,
- Suoyi Han,
- Suoyi Han,
- Suoyi Han,
- Suoyi Han,
- Suoyi Han,
- Du Wang,
- Muhammad Salman Haider,
- Junjia Guo,
- Junjia Guo,
- Junjia Guo,
- Junjia Guo,
- Qi Zhao,
- Qi Zhao,
- Qi Zhao,
- Pei Du,
- Pei Du,
- Pei Du,
- Pei Du,
- Pei Du,
- Ziqi Sun,
- Ziqi Sun,
- Ziqi Sun,
- Ziqi Sun,
- Feiyan Qi,
- Feiyan Qi,
- Feiyan Qi,
- Feiyan Qi,
- Zheng Zheng,
- Zheng Zheng,
- Zheng Zheng,
- Zheng Zheng,
- Bingyan Huang,
- Bingyan Huang,
- Bingyan Huang,
- Bingyan Huang,
- Wenzhao Dong,
- Wenzhao Dong,
- Wenzhao Dong,
- Wenzhao Dong,
- Peiwu Li,
- Xinyou Zhang,
- Xinyou Zhang,
- Xinyou Zhang,
- Xinyou Zhang,
- Xinyou Zhang
Affiliations
- Mengjie Cui
- College of Agriculture, Nanjing Agricultural University, Nanjing, China
- Mengjie Cui
- The Shennong Laboratory, Henan Academy of Crops Molecular Breeding, Henan Academy of Agricultural Science, Zhengzhou, China
- Mengjie Cui
- Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture, Zhengzhou, China
- Mengjie Cui
- Henan Provincial Key Laboratory for Oil Crop Improvement, Zhengzhou, China
- Mengjie Cui
- National Centre for Plant Breeding, Xinxiang, China
- Suoyi Han
- College of Agriculture, Nanjing Agricultural University, Nanjing, China
- Suoyi Han
- The Shennong Laboratory, Henan Academy of Crops Molecular Breeding, Henan Academy of Agricultural Science, Zhengzhou, China
- Suoyi Han
- Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture, Zhengzhou, China
- Suoyi Han
- Henan Provincial Key Laboratory for Oil Crop Improvement, Zhengzhou, China
- Suoyi Han
- National Centre for Plant Breeding, Xinxiang, China
- Du Wang
- Key Laboratory of Detection for Mycotoxins, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Wuhan, China
- Muhammad Salman Haider
- Department of Horticulture, Ghazi University, Dera Ghazi Khan, Pakistan
- Junjia Guo
- The Shennong Laboratory, Henan Academy of Crops Molecular Breeding, Henan Academy of Agricultural Science, Zhengzhou, China
- Junjia Guo
- Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture, Zhengzhou, China
- Junjia Guo
- Henan Provincial Key Laboratory for Oil Crop Improvement, Zhengzhou, China
- Junjia Guo
- National Centre for Plant Breeding, Xinxiang, China
- Qi Zhao
- The Shennong Laboratory, Henan Academy of Crops Molecular Breeding, Henan Academy of Agricultural Science, Zhengzhou, China
- Qi Zhao
- Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture, Zhengzhou, China
- Qi Zhao
- Henan Provincial Key Laboratory for Oil Crop Improvement, Zhengzhou, China
- Pei Du
- College of Agriculture, Nanjing Agricultural University, Nanjing, China
- Pei Du
- The Shennong Laboratory, Henan Academy of Crops Molecular Breeding, Henan Academy of Agricultural Science, Zhengzhou, China
- Pei Du
- Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture, Zhengzhou, China
- Pei Du
- Henan Provincial Key Laboratory for Oil Crop Improvement, Zhengzhou, China
- Pei Du
- National Centre for Plant Breeding, Xinxiang, China
- Ziqi Sun
- The Shennong Laboratory, Henan Academy of Crops Molecular Breeding, Henan Academy of Agricultural Science, Zhengzhou, China
- Ziqi Sun
- Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture, Zhengzhou, China
- Ziqi Sun
- Henan Provincial Key Laboratory for Oil Crop Improvement, Zhengzhou, China
- Ziqi Sun
- National Centre for Plant Breeding, Xinxiang, China
- Feiyan Qi
- The Shennong Laboratory, Henan Academy of Crops Molecular Breeding, Henan Academy of Agricultural Science, Zhengzhou, China
- Feiyan Qi
- Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture, Zhengzhou, China
- Feiyan Qi
- Henan Provincial Key Laboratory for Oil Crop Improvement, Zhengzhou, China
- Feiyan Qi
- National Centre for Plant Breeding, Xinxiang, China
- Zheng Zheng
- The Shennong Laboratory, Henan Academy of Crops Molecular Breeding, Henan Academy of Agricultural Science, Zhengzhou, China
- Zheng Zheng
- Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture, Zhengzhou, China
- Zheng Zheng
- Henan Provincial Key Laboratory for Oil Crop Improvement, Zhengzhou, China
- Zheng Zheng
- National Centre for Plant Breeding, Xinxiang, China
- Bingyan Huang
- The Shennong Laboratory, Henan Academy of Crops Molecular Breeding, Henan Academy of Agricultural Science, Zhengzhou, China
- Bingyan Huang
- Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture, Zhengzhou, China
- Bingyan Huang
- Henan Provincial Key Laboratory for Oil Crop Improvement, Zhengzhou, China
- Bingyan Huang
- National Centre for Plant Breeding, Xinxiang, China
- Wenzhao Dong
- The Shennong Laboratory, Henan Academy of Crops Molecular Breeding, Henan Academy of Agricultural Science, Zhengzhou, China
- Wenzhao Dong
- Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture, Zhengzhou, China
- Wenzhao Dong
- Henan Provincial Key Laboratory for Oil Crop Improvement, Zhengzhou, China
- Wenzhao Dong
- National Centre for Plant Breeding, Xinxiang, China
- Peiwu Li
- Key Laboratory of Detection for Mycotoxins, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Ministry of Agriculture and Rural Affairs, Wuhan, China
- Xinyou Zhang
- College of Agriculture, Nanjing Agricultural University, Nanjing, China
- Xinyou Zhang
- The Shennong Laboratory, Henan Academy of Crops Molecular Breeding, Henan Academy of Agricultural Science, Zhengzhou, China
- Xinyou Zhang
- Key Laboratory of Oil Crops in Huang-Huai-Hai Plains, Ministry of Agriculture, Zhengzhou, China
- Xinyou Zhang
- Henan Provincial Key Laboratory for Oil Crop Improvement, Zhengzhou, China
- Xinyou Zhang
- National Centre for Plant Breeding, Xinxiang, China
- DOI
- https://doi.org/10.3389/fpls.2022.899177
- Journal volume & issue
-
Vol. 13
Abstract
Cultivated peanut (Arachis hypogaea L.), a cosmopolitan oil crop, is susceptible to a variety of pathogens, especially Aspergillus flavus L., which not only vastly reduce the quality of peanut products but also seriously threaten food safety for the contamination of aflatoxin. However, the key genes related to resistance to Aspergillus flavus L. in peanuts remain unclear. This study identifies hub genes positively associated with resistance to A. flavus in two genotypes by comparative transcriptome and weighted gene co-expression network analysis (WGCNA) method. Compared with susceptible genotype (Zhonghua 12, S), the rapid response to A. flavus and quick preparation for the translation of resistance-related genes in the resistant genotype (J-11, R) may be the drivers of its high resistance. WGCNA analysis revealed that 18 genes encoding pathogenesis-related proteins (PR10), 1-aminocyclopropane-1-carboxylate oxidase (ACO1), MAPK kinase, serine/threonine kinase (STK), pattern recognition receptors (PRRs), cytochrome P450, SNARE protein SYP121, pectinesterase, phosphatidylinositol transfer protein, and pentatricopeptide repeat (PPR) protein play major and active roles in peanut resistance to A. flavus. Collectively, this study provides new insight into resistance to A. flavus by employing WGCNA, and the identification of hub resistance-responsive genes may contribute to the development of resistant cultivars by molecular-assisted breeding.
Keywords
- peanut
- Aspergillus flavus L.
- resistance
- transcriptome analysis
- weighted gene co-expression network analysis (WGCNA)
