International Journal of Hematology-Oncology and Stem Cell Research (Apr 2012)

Lymphoid Genes Expression in Mouse Embryonic Stem Cells with Differentiation on Feeder Free Condition

  • Najmaldin Saki,
  • Gholamreza Khamisipour,
  • Saeid Abroun,
  • Masoud Soleimani,
  • Mina Soufizomorrod,
  • Saeid Kaviani,
  • Majid Mossahebimohammadi

Journal volume & issue
Vol. 6, no. 2
pp. 22 – 26

Abstract

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Embryonic stem cells (ESCs) as pluripotent stem cells have the potential to give rise to all three germ layer cells. Also, these cells have the self-renewing ability. ESCs could differentiate into most of cell lineages. Lymphocytes generated from hematopoietic stem cells. We designed a feeder free culture condition to generate lymphoid lineage cells from ESCs. We demonstrated that IL-7 plays critical role in differentiation of ESCs into lymphoid lineage and expression of specific cell markers such as CD3, CD19 and CD25 after 14 days of differentiation by RT-PCR.

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