Design and construction of a low-cost compressive loading and perfusion flow bioreactor

Abstract

This article reports the design and construction of an open-source compressive loading and perfusion flow bioreactor for under $4000, as well as validation of the device and an example use-application. The bioreactor is capable of recording applied force and displacement as well as regulating media flow rate. This bioreactor was built to be user friendly, widely adaptable for modular changes, and made of readily available materials.

Keywords

• Bioreactor
• Hardware
• 3D printing
• Compressive loading
• Perfusion
• Osteogenesis