Clostridium butyricum Modulates the Microbiome to Protect Intestinal Barrier Function in Mice with Antibiotic-Induced Dysbiosis
Mao Hagihara,
Yasutoshi Kuroki,
Tadashi Ariyoshi,
Seiya Higashi,
Kazuo Fukuda,
Rieko Yamashita,
Asami Matsumoto,
Takeshi Mori,
Kaoru Mimura,
Naoko Yamaguchi,
Shoshiro Okada,
Tsunemasa Nonogaki,
Tadashi Ogawa,
Kenta Iwasaki,
Susumu Tomono,
Nobuhiro Asai,
Yusuke Koizumi,
Kentaro Oka,
Yuka Yamagishi,
Motomichi Takahashi,
Hiroshige Mikamo
Affiliations
Mao Hagihara
Department of Molecular Epidemiology and Biomedical Sciences, Aichi Medical University, Nagakute 480-1195, Japan
Yasutoshi Kuroki
Department of Clinical Infectious Diseases, Aichi Medical University, Nagakute 480-1195, Japan; Miyarisan Pharmaceutical Co., Ltd., Saitama 114-0016, Japan
Tadashi Ariyoshi
Department of Clinical Infectious Diseases, Aichi Medical University, Nagakute 480-1195, Japan; Miyarisan Pharmaceutical Co., Ltd., Saitama 114-0016, Japan
Seiya Higashi
Miyarisan Pharmaceutical Co., Ltd., Saitama 114-0016, Japan
Kazuo Fukuda
Miyarisan Pharmaceutical Co., Ltd., Saitama 114-0016, Japan
Rieko Yamashita
Department of Molecular Epidemiology and Biomedical Sciences, Aichi Medical University, Nagakute 480-1195, Japan
Asami Matsumoto
Miyarisan Pharmaceutical Co., Ltd., Saitama 114-0016, Japan
Takeshi Mori
Department of Clinical Infectious Diseases, Aichi Medical University, Nagakute 480-1195, Japan
Kaoru Mimura
Department of Pharmacology, Aichi Medical University, Nagakute 480-1195, Japan
Naoko Yamaguchi
Department of Pharmacology, Aichi Medical University, Nagakute 480-1195, Japan
Shoshiro Okada
Department of Pharmacology, Aichi Medical University, Nagakute 480-1195, Japan
Tsunemasa Nonogaki
Department of Pharmacy, College of Pharmacy Kinjo Gakuin University, Nagoya 463-8521, Japan
Tadashi Ogawa
Department of Legal Medicine, Aichi Medical University, Nagakute 480-1195, Japan
Kenta Iwasaki
Departments of Kidney Disease and Transplant Immunology, Aichi Medical University, Nagakute 480-1195, Japan
Susumu Tomono
Department of Microbiology and Immunology, Aichi Medical University, Nagakute 480-1195, Japan
Nobuhiro Asai
Department of Clinical Infectious Diseases, Aichi Medical University, Nagakute 480-1195, Japan
Yusuke Koizumi
Department of Clinical Infectious Diseases, Aichi Medical University, Nagakute 480-1195, Japan
Kentaro Oka
Department of Clinical Infectious Diseases, Aichi Medical University, Nagakute 480-1195, Japan; Miyarisan Pharmaceutical Co., Ltd., Saitama 114-0016, Japan
Yuka Yamagishi
Department of Clinical Infectious Diseases, Aichi Medical University, Nagakute 480-1195, Japan
Motomichi Takahashi
Department of Clinical Infectious Diseases, Aichi Medical University, Nagakute 480-1195, Japan; Miyarisan Pharmaceutical Co., Ltd., Saitama 114-0016, Japan
Hiroshige Mikamo
Department of Clinical Infectious Diseases, Aichi Medical University, Nagakute 480-1195, Japan; Corresponding author
Summary: Clostridium butyricum MIYAIRI 588 (CBM 588) is a probiotic bacterium that has previously been used to prevent antibiotic-associated diarrhea. However, the underlying mechanism by which CBM 588 protects the gut epithelial barrier remains unclear. Here, we show that CBM 588 increased the abundance of Bifidobacterium, Lactobacillus, and Lactococcus species in the gut microbiome and also enhanced the intestinal barrier function of mice with antibiotic-induced dysbiosis. Additionally, CBM 588 significantly promoted the expansion of IL-17A-producing γδT cells and IL-17A-producing CD4 cells in the colonic lamina propria (cLP), which was closely associated with changes in the intestinal microbial composition. Additionally, CBM 588 plays an important role in controlling antibiotic-induced gut inflammation through upregulation of anti-inflammatory lipid metabolites such as palmitoleic acid, 15d-prostaglandin J2, and protectin D1. This study reveals a previously unrecognized mechanism of CBM 588 and provides new insights into gut epithelial barrier protection with probiotics under conditions of antibiotic-induced dysbiosis. : Microbiome; Clinical Microbiology Subject Areas: Microbiome, Clinical Microbiology