Evaluation of beta-hemolytic, metallic green sheen, and ONPG test properties Escherichia coli isolated from urinary tract infections

Abstract

Introduction: Uropathogenic Escherichia coli strains are the most common cause of urinary tract infections in nosocomial and community-acquired infections. Phenotypic characteristics of Escherichia coli isolates in patients with urinary tract infections vary from region to region. Therefore, studying the phenotypic properties of the bacterium is very important. Materials and Methods: In the current study, 100 strains of Escherichia coli were detected from urine samples of patients with urinary tract infections in Mazandaran province, Babol. This study aimed to investigate the properties of metallic green sheen, beta hemolysis, and Ortho-nitrophenyl-β-D-galactopyranoside (ONPG) test of Escherichia coli. Results: The most common bacterium isolated from urinary tract infections was E. coli (68.02%). In the present study, the properties of beta hemolysis, metallic green sheen, and ONPG in uropathogenic E. coli were 1, 80, and 100%, respectively. Conclusion: The results of this study showed that 20% of E. coli strains lacked metallic green sheen, which should be identified through the IMViC test and other biochemical tests.

Keywords

• uropathogenic
• e. coli
• onpg test
• metallic green sheen
• β hemolysis