Journal of Inflammation Research (Mar 2022)

The Mutation of BTG2 Gene Predicts a Poor Outcome in Primary Testicular Diffuse Large B-Cell Lymphoma

  • Guo D,
  • Hong L,
  • Ji H,
  • Jiang Y,
  • Lu L,
  • Wang X,
  • Huang H

Journal volume & issue
Vol. Volume 15
pp. 1757 – 1769

Abstract

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Dan Guo,1,* Lemin Hong,1,* Hao Ji,2,* Yuwen Jiang,1 Ling Lu,1 Xinfeng Wang,1 Hongming Huang1 1Department of Hematology, The Affiliated Hospital of Nantong University, Jiangsu, People’s Republic of China; 2Department of Urology, Tumor Hospital Affiliated to Nantong University, Nantong, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xinfeng Wang; Hongming Huang, Department of Hematology, The Affiliated Hospital of Nantong University, No. 20, Xisi Street, Nantong, 226001, Jiangsu, People’s Republic of China, Email [email protected]; [email protected]: Primary testicular diffuse large B-cell lymphoma (PT-DLBCL) is a rare and aggressive form of mature B-cell lymphoma commonly found in elder males, but its genetic features are poorly understood. In this study, we had performed target-sequencing of 360 lymphoma-related genes on 76 PT-DLBCL patients with a median age of 65 (33– 89). Our data provide a comprehensive understanding of the landscape of mutations in a small subset of PT-DLBCL.Methods: A total of 76 PT-DLBCL patients were sequenced, and their clinical data and follow-up data were collected. The relationship between mutated genes, clinical data and prognosis and survival of PT-DLBCL patients was retrospectively analyzed by statistical software.Results: We observed a median of 15 protein-altering variants per patient in our data and was identified recurrent oncogenic mutations of 360 lymphoma-related genes involved in PT-DLBCL, including PIM1 (74%), MYD88 (50%), KMT2D (38%), KMT2C (34%), BTG2 (34%), TBL1XR1 (34%) and ETV6 (24%). Compared with classic DLBCL, PT-DLBCL showed an increased mutation frequency of PIM1, MYD88, BTG2, while NOTCH1 appeared exclusive mutated with PIM1, MSH3 and ETV6. Cox risk model regression analysis showed that age ≥ 60 years, IPI 3– 5 points, BTG2 gene mutation and extranodal organ invasion suggested poor prognosis. Finally, we constructed an OS predict model of PT-DLBCL patients using above factors with a high accuracy.Conclusion: In conclusion, our results revealed genomic characterization of PT-DLBCL, and the mutation of BTG2 was an independent factor predicting a poor prognosis.Keywords: primary testicular diffuse large B-cell lymphoma, genetic mutation, BTG2, prognosis, survival

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