Guoji Yanke Zazhi (Apr 2014)

Protective effects of lomerizine on retinal ganglion cells in early diabetic rats

  • Rui-Dong Gu,
  • Ying-Ying Hao,
  • Xiao-Long Chen

DOI
https://doi.org/10.3980/j.issn.1672-5123.2014.04.05
Journal volume & issue
Vol. 14, no. 4
pp. 593 – 598

Abstract

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AIM: To investigate the neuroprotection of lomerizine(LOM)on retinal ganglion cells(RGCs)in early diabetic rats and their mechanism.METHODS: Adult male Spraque - Dawley(SD)rats were randomly divided into control(CON), diabetes mellitus(DM), lomerizine(LOM)group, and each group had 40 rats. Diabetes rat model was induced by Streptozotocin(STZ)of intraperitoneal injection of 60 mg/kg of disposable. In LOM group, after model was established, rats were lavaged LOM by the dosage of 60mg/kg daily, the CON group and DM group were given the same dosage sodium chloride. In 4th, 8th, 12th wk, RGCs' apoptosis were detected by HE, TUNEL, transmission electron microscope, and TUNEL and laser confocal microscope detection was used to test the calcium ion concentration.RESULTS: Morphological observation: with the extension of the DM, RGCs decreased gradually and appeared disordered arrangement of cells. In DM group, different stages of apoptosis were observed by transmission electron microscope and got worse gradually with its extension. In LOM group, compared with DM group in the same period, RGCs apoptosis signs diminished gradually at 8th and 12th wk. TUNEL detection: no apoptotic RGCs was observed in CON group. In DM group, few TUNEL positive RGCs were seen at 4th wk, and became more and more gradually. The apoptosis index was significantly higher in DM group compared with CON group in same time and there was statistical significance(Pth and 12th wk, compared with DM group in the same period, the numbers of TUNEL positive RGCs decreased. The apoptosis index was significantly lower in LOM group compared with DM group in the same period(Pth and 12th week, DM group's calcium fluorescent staining intensity of RGCs markedly elevated and had significant differences.(Pth and 12th wk, calcium fluorescent staining intensity of RGCs markedly decreased and had statistical significance(PCONCLUSION: The LOM played a protective role for RGCs in early stage of diabetic rats.

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