HPLC-DAD-ESI-MS profile, antibacterial activity, and modulation of the activity of antibiotics by Carica papaya L. against Escherichia coli serotypes

Cícera Natalia Figueiredo Leite Gondim; Denise Aline Casimiro Bezerra; Nadghia Figueiredo Leite Sampaio; Maria Anésia Sousa de.Alencar; Roger Henrique Sousa da.Costa; Lucas Silva Abreu; Joanda Paolla Raimundo.e.Silva; Francisco Nascimento Pereira Junior; Josean Fechine Tavares; Marcelo Sobral da Silva; Jaime Ribeiro-Filho; Henrique Douglas Melo Coutinho

Phytomedicine Plus (Aug 2022)

HPLC-DAD-ESI-MS profile, antibacterial activity, and modulation of the activity of antibiotics by Carica papaya L. against Escherichia coli serotypes

Cícera Natalia Figueiredo Leite Gondim,
Denise Aline Casimiro Bezerra,
Nadghia Figueiredo Leite Sampaio,
Maria Anésia Sousa de.Alencar,
Roger Henrique Sousa da.Costa,
Lucas Silva Abreu,
Joanda Paolla Raimundo.e.Silva,
Francisco Nascimento Pereira Junior,
Josean Fechine Tavares,
Marcelo Sobral da Silva,
Jaime Ribeiro-Filho,
Henrique Douglas Melo Coutinho

Affiliations

Cícera Natalia Figueiredo Leite Gondim: Student of the Postgraduate in Ethnobiology and Nature Conservation, Federal Rural University of Pernambuco – UFRPE, Recife, PE, Brazil
Denise Aline Casimiro Bezerra: Laboratory of Microbiology and Molecular Biology – LMBM, Regional University of Cariri URCA Crato, CE, Brazil
Nadghia Figueiredo Leite Sampaio: Student of the Postgraduate in Biological Chemistry at the Regional University of Cariri URCA Crato, CE, Brazil
Maria Anésia Sousa de.Alencar: Laboratory of Microbiology and Molecular Biology – LMBM, Regional University of Cariri URCA Crato, CE, Brazil
Roger Henrique Sousa da.Costa: Student of the Postgraduate in Ethnobiology and Nature Conservation, Federal Rural University of Pernambuco – UFRPE, Recife, PE, Brazil
Lucas Silva Abreu: Professor of the Postgraduate in Chemistry, Fluminense Federal University Rio de Janeiro, RJ, Brazil
Joanda Paolla Raimundo.e.Silva: Professor of the Postgraduate in Chemistry, Fluminense Federal University Rio de Janeiro, RJ, Brazil
Francisco Nascimento Pereira Junior: Professor of the Postgraduate in Natural and Bioactive Synthetic Products, Federal University of Paraíba João Pessoa, PB. Brazil; Professor of the Federal University of Cariri, Crato, CE, Brazil
Josean Fechine Tavares: Professor of the Postgraduate in Natural and Bioactive Synthetic Products, Federal University of Paraíba João Pessoa, PB. Brazil
Marcelo Sobral da Silva: Professor of the Postgraduate in Natural and Bioactive Synthetic Products, Federal University of Paraíba João Pessoa, PB. Brazil
Jaime Ribeiro-Filho: Researcher at the Goncalo Moniz Institute (IGM), Oswaldo Cruz Foundation, Salvador, BA, Brazil
Henrique Douglas Melo Coutinho: Professor of the Postgraduate in Ethnobiology and Nature Conservation at the Federal Rural University of Pernambuco – UFRPE. Recife, PE, Brazil; Laboratory of Microbiology and Molecular Biology – LMBM, Regional University of Cariri URCA Crato, CE, Brazil; Corresponding author.

Journal volume & issue: Vol. 2, no. 3
p. 100306

Abstract

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Background: Diarrhea is currently the second leading cause of death among children under five years old in the world and therefore, remains a global public health problem. Considering that most cases of diarrhea are due to gastrointestinal infections, and medicinal plants have been traditionally used to treat such conditions, this work aimed to evaluate the chemical composition, antibacterial activity, and antibiotic-enhancing properties of C. papaya against Escherichia coli, EPEC (Enteropathogenic E. coli), and ETEC (Enterotoxigenic E. coli) serotypes.Methods: The chemical profile of the aqueous extract obtained from C. papaya leaves was determined by HPLC-DAD-ESI-MS. The antibacterial and antibiotic-enhancing activities were evaluated through the determination of the MIC, following the microdilution method.Results: A total of 17 compounds were identified by HPLC-DAD-ESI-MS. However, the extract of C. papaya did not present an intrinsic antibacterial effect, in addition to showing no modulatory effect when associated with antibiotics.Conclusions: Despite the presence of bioactive compounds, the aqueous extract C. papaya does not present antibacterial or antibiotic-enhancing effects against the ETEC and EPEC strains of E. coli. Nevertheless, further research is required to determine if its isolated components are potential drug candidates for the therapy of infections caused by these strains.

Published in Phytomedicine Plus

ISSN: 2667-0313 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Medicine: Other systems of medicine
Website: https://www.journals.elsevier.com/phytomedicine-plus/

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