Dynamics of Auxin and Cytokinin Metabolism during Early Root and Hypocotyl Growth in <i>Theobroma cacao</i>
Alexandre Mboene Noah,
Rubén Casanova-Sáez,
Rolande Eugenie Makondy Ango,
Ioanna Antoniadi,
Michal Karady,
Ondřej Novák,
Nicolas Niemenak,
Karin Ljung
Affiliations
Alexandre Mboene Noah
Department of Biochemistry, Faculty of Science, University of Douala, Douala P.O. Box 24157, Cameroon
Rubén Casanova-Sáez
Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences (SLU), 90183 Umeå, Sweden
Rolande Eugenie Makondy Ango
Laboratory of Plant Physiology and Biochemistry, Department of Biological Science, Higher Teachers’ Training College, University of Yaounde I, Yaounde P.O. Box 47, Cameroon
Ioanna Antoniadi
Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences (SLU), 90183 Umeå, Sweden
Michal Karady
Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences (SLU), 90183 Umeå, Sweden
Ondřej Novák
Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences (SLU), 90183 Umeå, Sweden
Nicolas Niemenak
Laboratory of Plant Physiology and Biochemistry, Department of Biological Science, Higher Teachers’ Training College, University of Yaounde I, Yaounde P.O. Box 47, Cameroon
Karin Ljung
Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences (SLU), 90183 Umeå, Sweden
The spatial location and timing of plant developmental events are largely regulated by the well balanced effects of auxin and cytokinin phytohormone interplay. Together with transport, localized metabolism regulates the concentration gradients of their bioactive forms, ultimately eliciting growth responses. In order to explore the dynamics of auxin and cytokinin metabolism during early seedling growth in Theobroma cacao (cacao), we have performed auxin and cytokinin metabolite profiling in hypocotyls and root developmental sections at different times by using ultra-high-performance liquid chromatography-electrospray tandem mass spectrometry (UHPLC-MS/MS). Our work provides quantitative characterization of auxin and cytokinin metabolites throughout early root and hypocotyl development and identifies common and distinctive features of auxin and cytokinin metabolism during cacao seedling development.
