Insights into the physico-chemical and biological characterization of sodium lignosulfonate - silver nanosystems designed for wound management
Ioana C. Marinas,
Leonard Ignat,
Ignat E. Maurușa,
Madalina D. Gaboreanu,
Coroabă Adina,
Marcela Popa,
Mariana C. Chifiriuc,
Marian Angheloiu,
Mihaela Georgescu,
Alexandra Iacobescu,
Gratiela Gradisteanu Pircalabioru,
Miruna Stan,
Mariana Pinteala
Affiliations
Ioana C. Marinas
Research Institute of the University of Bucharest—ICUB, University of Bucharest, 050095, Bucharest, Romania; Research and Development Department of SC Sanimed International Impex SRL, 6 Bucharest -Giurgiu Street, 087040, Giurgiu, Romania
Leonard Ignat
Centre of Advanced Research in Bionanoconjugates and Biopolymers, “Petru Poni” Institute of Macromolecular Chemistry, Gr. Ghica Voda Alley 41A, Iasi, 700487, Romania; Corresponding author.
Ignat E. Maurușa
Centre of Advanced Research in Bionanoconjugates and Biopolymers, “Petru Poni” Institute of Macromolecular Chemistry, Gr. Ghica Voda Alley 41A, Iasi, 700487, Romania
Madalina D. Gaboreanu
Research Institute of the University of Bucharest—ICUB, University of Bucharest, 050095, Bucharest, Romania; Faculty of Biology, Department of Botany and Microbiology, University of Bucharest, 1-3 Portocalelor Street, 060101, Bucharest, Romania
Coroabă Adina
Centre of Advanced Research in Bionanoconjugates and Biopolymers, “Petru Poni” Institute of Macromolecular Chemistry, Gr. Ghica Voda Alley 41A, Iasi, 700487, Romania
Marcela Popa
Research Institute of the University of Bucharest—ICUB, University of Bucharest, 050095, Bucharest, Romania; Faculty of Biology, Department of Botany and Microbiology, University of Bucharest, 1-3 Portocalelor Street, 060101, Bucharest, Romania
Mariana C. Chifiriuc
Research Institute of the University of Bucharest—ICUB, University of Bucharest, 050095, Bucharest, Romania; Faculty of Biology, Department of Botany and Microbiology, University of Bucharest, 1-3 Portocalelor Street, 060101, Bucharest, Romania; Romanian Academy of Scientists, 54 Spl. Independentei St., District 5, 50085, Bucharest, Romania; The Romanian Academy, 25, Calea Victoriei, Sector 1, District 1, 010071, Bucharest, Romania; Corresponding author. Research Institute of the University of Bucharest—ICUB, University of Bucharest, 050095, Bucharest, Romania.
Marian Angheloiu
Research and Development Department of SC Sanimed International Impex SRL, 6 Bucharest -Giurgiu Street, 087040, Giurgiu, Romania
Mihaela Georgescu
Research Institute of the University of Bucharest—ICUB, University of Bucharest, 050095, Bucharest, Romania
Alexandra Iacobescu
Centre of Advanced Research in Bionanoconjugates and Biopolymers, “Petru Poni” Institute of Macromolecular Chemistry, Gr. Ghica Voda Alley 41A, Iasi, 700487, Romania
Gratiela Gradisteanu Pircalabioru
Research Institute of the University of Bucharest—ICUB, University of Bucharest, 050095, Bucharest, Romania; Faculty of Biology, Department of Botany and Microbiology, University of Bucharest, 1-3 Portocalelor Street, 060101, Bucharest, Romania
Miruna Stan
Research Institute of the University of Bucharest—ICUB, University of Bucharest, 050095, Bucharest, Romania; Faculty of Biology, Department of Biochemistry and Molecular Biology, University of Bucharest, 91-95 Splaiul Independentei, 050095, Bucharest, Romania
Mariana Pinteala
Centre of Advanced Research in Bionanoconjugates and Biopolymers, “Petru Poni” Institute of Macromolecular Chemistry, Gr. Ghica Voda Alley 41A, Iasi, 700487, Romania
Chronic wounds represent one of the complications that might occur from the disruption of wound healing process. Recently, there has been a rise in interest in employing nanotechnology to develop novel strategies for accelerating wound healing. The aim of the present study was to use a green synthesis method to obtain AgNPs/NaLS systems useful for wounds management and perform an in-depth investigation of their behavior during and post-synthesis as well as of their biological properties. The colloids obtained from silver nanoparticles (AgNPs) and commercial sodium lignosulfonate (NaLS) in a single-pot aqueous procedure have been fully characterized by UV–Vis, FT-IR, DLS, TEM, XRD, and XPS to evaluate the synthesis efficiency and to provide new insights in the process of AgNPs formation and NaLS behavior in aqueous solutions. The effects of various concentrations of NaLS (0–16 mg/mL) and AgNO3 (0–20 mM) and of two different temperatures on AgNPs formation have been analyzed. Although the room temperature is feasible for AgNPs synthesis, the short mixing at 70 °C significantly increases the speed of nanoparticle formation and storage stability. In all experimental conditions AgNPs of 20–40 nm in size have been obtained. The antimicrobial activity assessed quantitatively on clinical and reference bacterial strains, both in suspension and biofilm growth state, revealed a broad antimicrobial spectrum, the most intensive inhibitory effect being noticed against Pseudomonas aeruginosa and Escherichia coli strains. The AgNP/NaLS enhanced the NO extracellular release, potentially contributing to the microbicidal and anti-adherence activity by protein oxidation. Both AgNP/NaLS and NaLS were non-hemolytic (hemolytic index<5%, 2.26 ± 0.13% hemolysis) and biocompatible (102.17 ± 3.43 % HaCaT cells viability). The presence of AgNPs increased the antioxidative activity and induced a significant cytotoxicity on non-melanoma skin cancer cells (62.86 ± 8.27% Cal-27 cells viability). Taken together, all these features suggest the multivalent potential of these colloids for the development of novel strategies for wound management, acting by preventing infection-associated complications and supporting the tissue regeneration.
