Frontiers in Immunology (Nov 2024)
Succession of the multi-site microbiome along pancreatic ductal adenocarcinoma tumorigenesis
- Yiqing Zhu,
- Yiqing Zhu,
- Yiqing Zhu,
- Yiqing Zhu,
- Xiao Liang,
- Xiao Liang,
- Xiao Liang,
- Xiao Liang,
- Mengfan Zhi,
- Mengfan Zhi,
- Lixiang Li,
- Lixiang Li,
- Lixiang Li,
- Lixiang Li,
- Guoming Zhang,
- Guoming Zhang,
- Guoming Zhang,
- Guoming Zhang,
- Changxu Chen,
- Changxu Chen,
- Changxu Chen,
- Changxu Chen,
- Limei Wang,
- Limei Wang,
- Limei Wang,
- Limei Wang,
- Peng Wang,
- Peng Wang,
- Peng Wang,
- Peng Wang,
- Ning Zhong,
- Ning Zhong,
- Ning Zhong,
- Ning Zhong,
- Qiang Feng,
- Qiang Feng,
- Zhen Li,
- Zhen Li,
- Zhen Li,
- Zhen Li
Affiliations
- Yiqing Zhu
- Department of Gastroenterology, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Yiqing Zhu
- Shandong Provincial Clinical Research Center for Digestive Disease, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Yiqing Zhu
- Laboratory of Translational Gastroenterology, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Yiqing Zhu
- Robot Engineering Laboratory for Precise Diagnosis and Therapy of Gastrointestinal (GI) Tumor, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Xiao Liang
- Department of Gastroenterology, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Xiao Liang
- Shandong Provincial Clinical Research Center for Digestive Disease, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Xiao Liang
- Laboratory of Translational Gastroenterology, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Xiao Liang
- Robot Engineering Laboratory for Precise Diagnosis and Therapy of Gastrointestinal (GI) Tumor, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Mengfan Zhi
- Shandong Key Laboratory of Oral Tissue Regeneration, Department of Human Microbiome, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China
- Mengfan Zhi
- Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases, Shandong University, Jinan, Shandong, China
- Lixiang Li
- Department of Gastroenterology, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Lixiang Li
- Shandong Provincial Clinical Research Center for Digestive Disease, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Lixiang Li
- Laboratory of Translational Gastroenterology, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Lixiang Li
- Robot Engineering Laboratory for Precise Diagnosis and Therapy of Gastrointestinal (GI) Tumor, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Guoming Zhang
- Department of Gastroenterology, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Guoming Zhang
- Shandong Provincial Clinical Research Center for Digestive Disease, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Guoming Zhang
- Laboratory of Translational Gastroenterology, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Guoming Zhang
- Robot Engineering Laboratory for Precise Diagnosis and Therapy of Gastrointestinal (GI) Tumor, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Changxu Chen
- Department of Gastroenterology, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Changxu Chen
- Shandong Provincial Clinical Research Center for Digestive Disease, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Changxu Chen
- Laboratory of Translational Gastroenterology, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Changxu Chen
- Robot Engineering Laboratory for Precise Diagnosis and Therapy of Gastrointestinal (GI) Tumor, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Limei Wang
- Department of Gastroenterology, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Limei Wang
- Shandong Provincial Clinical Research Center for Digestive Disease, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Limei Wang
- Laboratory of Translational Gastroenterology, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Limei Wang
- Robot Engineering Laboratory for Precise Diagnosis and Therapy of Gastrointestinal (GI) Tumor, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Peng Wang
- Department of Gastroenterology, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Peng Wang
- Shandong Provincial Clinical Research Center for Digestive Disease, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Peng Wang
- Laboratory of Translational Gastroenterology, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Peng Wang
- Robot Engineering Laboratory for Precise Diagnosis and Therapy of Gastrointestinal (GI) Tumor, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Ning Zhong
- Department of Gastroenterology, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Ning Zhong
- Shandong Provincial Clinical Research Center for Digestive Disease, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Ning Zhong
- Laboratory of Translational Gastroenterology, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Ning Zhong
- Robot Engineering Laboratory for Precise Diagnosis and Therapy of Gastrointestinal (GI) Tumor, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Qiang Feng
- Shandong Key Laboratory of Oral Tissue Regeneration, Department of Human Microbiome, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University, Jinan, Shandong, China
- Qiang Feng
- Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases, Shandong University, Jinan, Shandong, China
- Zhen Li
- Department of Gastroenterology, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Zhen Li
- Shandong Provincial Clinical Research Center for Digestive Disease, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Zhen Li
- Laboratory of Translational Gastroenterology, Qilu Hospital of Shandong University, Jinan, Shandong, China
- Zhen Li
- Robot Engineering Laboratory for Precise Diagnosis and Therapy of Gastrointestinal (GI) Tumor, Qilu Hospital of Shandong University, Jinan, Shandong, China
- DOI
- https://doi.org/10.3389/fimmu.2024.1487242
- Journal volume & issue
-
Vol. 15
Abstract
BackgroundTo investigate microbial characteristics across multibody sites from chronic pancreatitis (CP), through pancreatic benign tumors, to pancreatic ductal adenocarcinoma (PDAC) at different stages.Methods16S ribosomal RNA (rRNA) amplicon sequencing was conducted on saliva, duodenal fluid, and pancreatic tissue obtained via endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) of patients with CP, pancreatic benign tumors, PDAC in stage I/II, III, and IV. The neutral community model (NCM) assessed the microbial assembly contribution and MaAslin2 identified the differential microbes.ResultsFrom CP to stage IV PDAC patients, there was a marked surge in influence of salivary and duodenal microbiota on constitution of pancreatic microbial communities. Our observations revealed a successive alteration in microbial species across various bodily sites during PDAC tumorigenesis. Notably, Porphyromonas gingivalis, Treponema denticola, Peptoanaerobacter stomatis, Propionibacterium acidifaciens, Porphyromonas endodontalis, Filifactor alocis, etc., sequentially increased along PDAC progression in pancreatic tissue, whereas bacteria commonly used as probiotics Bifidobacterium breve, Lactiplantibacillus plantarum, etc., declined. Furthermore, the sequentially escalating trends of Peptoanaerobacter stomatis and Propionibacterium acidifaciens during PDAC tumorigenesis were mirrored in duodenal fluid and saliva. Porphyromonas gingivalis, Porphyromonas endodontalis, and Filifactor alocis, which intensified from CP to stage IV PDAC in pancreatic tissue, were also found to be enriched in saliva of patients with short-term survival (STS) compared with those with long-term survival (LTS).ConclusionsSalivary and duodenal microorganisms were prominent factors in shaping pancreatic microbial landscape in PDAC context. Further exploration of these microbial entities is imperative to unravel their specific roles in PDAC pathogenesis, potentially yielding insights for future therapeutic strategies.
Keywords
- pancreatic neoplasms
- pancreatitis
- endoscopic ultrasound-guided fine needle aspiration
- microbiota
- time series analysis
