Oleuropein as An Effective Suppressor of Inflammation and MicroRNA-146a Expression in Patients with Rheumatoid Arthritis

Hanieh Mahi; Zahra Yousefi; Fatemeh Toufan; Maryam Yarmohammadi; Moslem Jafarisani; Nahid Eskandari; Mohammad- Hossein Shams; Reza Jafari

doi:10.22074/cellj.2023.1989941.1237

Cell Journal (Jul 2023)

Oleuropein as An Effective Suppressor of Inflammation and MicroRNA-146a Expression in Patients with Rheumatoid Arthritis

Hanieh Mahi,
Zahra Yousefi,
Fatemeh Toufan,
Maryam Yarmohammadi,
Moslem Jafarisani,
Nahid Eskandari,
Mohammad- Hossein Shams,
Reza Jafari

Affiliations

Hanieh Mahi: Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
Zahra Yousefi: Schools of Allied Medical Sciences, Shahroud University of Medical Sciences, Shahroud, Iran
Fatemeh Toufan: Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
Maryam Yarmohammadi: Student Research Committee, School of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran
Moslem Jafarisani: Schools of Allied Medical Sciences, Shahroud University of Medical Sciences, Shahroud, Iran
Nahid Eskandari: Department of Immunology, Isfahan University of Medical Sciences, Isfahan, Iran
Mohammad- Hossein Shams: Department of Medical Immunology, School of Medicine, Lorestan University of Medical Sciences, Khorramabad, Iran
Reza Jafari: Schools of Allied Medical Sciences, Shahroud University of Medical Sciences, Shahroud, Iran

DOI: https://doi.org/10.22074/cellj.2023.1989941.1237
Journal volume & issue: Vol. 25, no. 7
pp. 505 – 512

Abstract

Read online

Objective: Rheumatoid arthritis (RA) is a common progressive autoimmune disorder that causes chronic inflammationof the joints and damage to other organs. Previous studies have reported the important role of miRNA-146a in thepathogenesis of RA. In addition, the anti-inflammatory and modulatory effects of oleuropein (OLEU) on the expressionpattern of microRNAs (miRNAs) have been shown in different diseases. Therefore, this study aimed to evaluate boththe sensitivity and specificity of miRNA-146a and determine the potential effects of OLEU on the expression levels ofmiRNA-146a and tumour necrosis factor-alpha (TNF-α) in RA patients.Materials and Methods: The participants in this experimental study were divided into 2 groups: RA (n=45) and healthycontrols (n=30). The isolated peripheral blood mononuclear cells (PBMCs) were treated with different concentrationsof OLEU; and the level of TNF-α expression, anti-citrullinated protein, and miRNA-146a were determined usingenzyme-linked immunoassay and real-time polymerase chain reaction, respectively. In addition, the receiver operatingcharacteristic (ROC) curve analysis evaluated the sensitivity and specificity of miRNA-146a in RA patients.Results: Results revealed a positive correlation between the levels of miRNA-146a expression with the serum levels ofC-reactive protein (CRP) and rheumatoid factor (RF) in RA patients. In addition, OLEU treatment decreased the levelsof TNF-α and miRNA-146a expression in treated PBMCs samples compared with untreated cells. The ROC curveanalysis showed an 85% sensitivity and 100% specificity of miRNA-146a in RA patients.Conclusion: Therefore, miRNA-146a can be used as a useful biomarker for RA diagnosis, particularly for earlydetection. In addition, OLEU could suppress inflammation in RA patients through the regulation of miRNA-146a.

Published in Cell Journal

ISSN: 2228-5806 (Print); 2228-5814 (Online)
Publisher: Royan Institute (ACECR), Tehran
Country of publisher: Iran, Islamic Republic of
LCC subjects: Medicine; Science
Website: http://celljournal.org/

About the journal

Abstract

Keywords