A de novo reference transcriptome for Bolitoglossa vallecula, an Andean mountain salamander in Colombia
Claudia M. Arenas Gómez,
M. Ryan Woodcock,
Jeramiah J. Smith,
S. Randal Voss,
Jean Paul Delgado
Affiliations
Claudia M. Arenas Gómez
Universidad de Antioquia. Sede de Investigación Universitaria. Torre 2, laboratorio 432. Calle 62 No. 52 – 59. Medellín, Colombia; Current address: Marine Biological Laboratory, Eugene Bell Center for Regenerative Biology and Tissue Engineering, Woods Hole, 02543 MA, USA; Corresponding author. Universidad de Antioquia. Sede de Investigación Universitaria. Torre 2, laboratorio 432. Calle 62 No. 52 – 59. Medellín, Colombia.
M. Ryan Woodcock
Department of Science, Math, and Technology, Medaille College, Buffalo, NY, 14214, USA
Jeramiah J. Smith
Department of Biology, University of Kentucky. Lexington, KY 40506, USA
S. Randal Voss
Department of Neuroscience, Spinal Cord and Brain Injury Research Center, University of Kentucky. Lexington, KY 40536, USA
Jean Paul Delgado
Universidad de Antioquia. Sede de Investigación Universitaria. Torre 2, laboratorio 432. Calle 62 No. 52 – 59. Medellín, Colombia; Corresponding author.
The amphibian order Caudata, contains several important model species for biological research. However, there is need to generate transcriptome data from representative species of the primary salamander families. Here we describe a de novo reference transcriptome for a terrestrial salamander, Bolitoglossa vallecula (Caudata: Plethodontidae). We employed paired-end (PE) illumina RNA sequencing to assemble a de novo reference transcriptome for B. vallecula. Assembled transcripts were compared against sequences from other vertebrate taxa to identify orthologous genes, and compared to the transcriptome of a close plethodontid relative (Bolitoglossa ramosi) to identify commonly expressed genes in the skin. This dataset should be useful to future comparative studies aimed at understanding important biological process, such as immunity, wound healing, and the production of antimicrobial compounds. Keywords: Bolitoglossa, Plethodontid, Salamanders, Skin, Transcriptomics
