Exploring the Root Morphological Traits of Diverse-Origin Cultivated Soybean

Abstract

Root morphological traits (RMTs) profoundly influence plant growth, resilience to abiotic stresses, and yield in soybean (Glycine max). In a comprehensive study spanning two consecutive years (2021–2022), the RMTs were assessed in 216 soybean accessions from 34 diverse origins. The investigation involved randomized batches with plants cultivated in PVC pipes filled with horticultural soil and harvested at the V2 growth stage. All the germplasms exhibited significant differences (p r = 0.85), NT (r = 0.84), NF (r = 0.96), and TTL (r = 0.88), whereas it had a negative association with AD (r = −0.25). A principal component analysis (PCA) showed a cumulative 95% of the total variance in the data in the first three principal components (PCs). PC1 (eigenvalue = 4.64) accounted for a 77.00% variance, with TRL, RV, NF, NT, and TTL exhibiting the highest associated eigenvectors. K-means clustering was performed with three clusters. Cluster 2 contained accessions with higher AD values, whereas Cluster 3 comprised accessions with increased TRL, NT, NF, and TTL, which mostly originated from Korea. Our findings offer targeted insights for plant breeders to optimize specific root traits and enhance crop performance across diverse environmental conditions by strategically targeting these clusters. Additionally, the influence of cultivar origin on root traits warrants further investigation, with implications for future breeding programs.

Keywords

• soybean <i>(Glycine max)</i>
• germplasm
• root morphological traits (RMTs)
• plant breeding
• 2D root imaging
• WinRHIZO