‘Sultanina’ leaves increase their trehalose content in response to grapevine brown leaf spot infection by regulating the pentose and glucuronate interchange pathway
Chuan Zhang,
Haixia Zhong,
Haoyu Chen,
Nuerziya Yalimaimaiti,
Ju Liang,
Jiachen Duan,
Yameng Yang,
Songlin Zhang,
Vivek Yadav,
Xiaoming Zhou,
Xinyu Wu,
Fuchun Zhang,
Jingzhe Hao
Affiliations
Chuan Zhang
Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, PR China; Institute of Horticulture Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, PR China; The State Key Laboratory of Genetic Improvement and Germplasm Innovation of Crop Resistance in Arid Desert Regions (Preparation), Urumqi 830091, PR China; Key Laboratory of Genome Research and Genetic Improvement of Xinjiang Characteristic Fruits and Vegetables, Urumqi 830091, PR China
Haixia Zhong
Institute of Horticulture Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, PR China; The State Key Laboratory of Genetic Improvement and Germplasm Innovation of Crop Resistance in Arid Desert Regions (Preparation), Urumqi 830091, PR China; Key Laboratory of Genome Research and Genetic Improvement of Xinjiang Characteristic Fruits and Vegetables, Urumqi 830091, PR China
Haoyu Chen
Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, PR China
Nuerziya Yalimaimaiti
Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, PR China
Ju Liang
Turpan Institute of Agricultural Sciences, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, PR China
Jiachen Duan
Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, PR China
Yameng Yang
Luoyang Academy of Agriculture and Forestry Sciences, Luoyang 471023, PR China
Songlin Zhang
Institute of Horticulture Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, PR China; The State Key Laboratory of Genetic Improvement and Germplasm Innovation of Crop Resistance in Arid Desert Regions (Preparation), Urumqi 830091, PR China; Key Laboratory of Genome Research and Genetic Improvement of Xinjiang Characteristic Fruits and Vegetables, Urumqi 830091, PR China
Vivek Yadav
Institute of Horticulture Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, PR China; The State Key Laboratory of Genetic Improvement and Germplasm Innovation of Crop Resistance in Arid Desert Regions (Preparation), Urumqi 830091, PR China; Key Laboratory of Genome Research and Genetic Improvement of Xinjiang Characteristic Fruits and Vegetables, Urumqi 830091, PR China
Xiaoming Zhou
Institute of Horticulture Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, PR China; The State Key Laboratory of Genetic Improvement and Germplasm Innovation of Crop Resistance in Arid Desert Regions (Preparation), Urumqi 830091, PR China; Key Laboratory of Genome Research and Genetic Improvement of Xinjiang Characteristic Fruits and Vegetables, Urumqi 830091, PR China
Xinyu Wu
Institute of Horticulture Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, PR China; The State Key Laboratory of Genetic Improvement and Germplasm Innovation of Crop Resistance in Arid Desert Regions (Preparation), Urumqi 830091, PR China; Key Laboratory of Genome Research and Genetic Improvement of Xinjiang Characteristic Fruits and Vegetables, Urumqi 830091, PR China; Corresponding authors.
Fuchun Zhang
Institute of Horticulture Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, PR China; The State Key Laboratory of Genetic Improvement and Germplasm Innovation of Crop Resistance in Arid Desert Regions (Preparation), Urumqi 830091, PR China; Key Laboratory of Genome Research and Genetic Improvement of Xinjiang Characteristic Fruits and Vegetables, Urumqi 830091, PR China; Corresponding authors.
Jingzhe Hao
Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, PR China; Correspondence author at: Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, PR China.
Since the first discovery of grapevine brown leaf spot disease in Turpan, Xinjiang, China, in 2007, it has been a common occurrence in grapevine-growing regions. Grapevine brown leaf spot seriously decreases fruit yield and has become one of the most important leaf diseases in the Turpan region. However, thus far, there have been no reports on the evaluation of grapevine germplasm resources for resistance to brown leaf spot disease. In addition, the response mechanism of grapevine leaves to brown leaf spot infection has not been revealed. To better understand the resistance of grapevine germplasm resources to brown leaf spot disease, we first evaluated resistance in grapevine varieties. On this basis, the most susceptible variety ‘Sultanina’ was selected as the experimental material for this study. Transcriptome analysis and carbohydrate content analysis were performed on ‘Sultanina’ leaves with different levels of disease susceptibility. As the severity of the disease increased, the content of fructose gradually decreased, while the content of trehalose gradually increased. Transcriptome data revealed that differentially expressed genes were enriched in the pentose and glucuronate interchange pathway. These results suggest that the sugar trehalose may play an important role in the response of ‘Sultanina’ leaves to brown leaf spot infection. In addition, the pentose and glucuronate interchange pathway may be involved in the response mechanism of brown leaf spot disease. Our work provides new insights into the mechanism of the ‘Sultanina’ leaf response to brown leaf spot infection.
