Platelets (Dec 2023)

Platelet-derived mitochondria transfer facilitates wound-closure by modulating ROS levels in dermal fibroblasts

  • Soomin Kim,
  • Yujin Kim,
  • Shin-Hye Yu,
  • Seo-Eun Lee,
  • Jong Hyeok Park,
  • Gayoung Cho,
  • Chul Choi,
  • Kyuboem Han,
  • Chun-Hyung Kim,
  • Young Cheol Kang

DOI
https://doi.org/10.1080/09537104.2022.2151996
Journal volume & issue
Vol. 34, no. 1

Abstract

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Platelets are known to improve the wound-repair capacity of mesenchymal stem cells (MSCs) by transferring mitochondria intercellularly. This study aimed to investigate whether direct transfer of mitochondria (pl-MT) isolated from platelets could enhance wound healing in vitro using a cell-based model. Wound repairs were assessed by 2D gap closure experiment in wound scratch assay using human dermal fibroblasts (hDFs). Results demonstrated that pl-MT were successfully internalized into hDFs. It increased cell proliferation and promoted the closure of wound gap. Importantly, pl-MT suppressed both intracellular and mitochondrial ROS production induced by hydrogen peroxide, cisplatin, and TGF-β in hDFs. Taken together, these results suggest that pl-MT transfer might be used as a potential therapeutic strategy for wound repair.

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