Advancing CEA quantification: Designing a sensitive electrochemical immunosenor using MWCNT/Ni(OH)2 nanocomposite

Abstract

An ultra-sensitive immunosensor was designed for the accurate determination of Carcinoembryonic Antigen (CEA). To enhance the performance of immunosensor, an MWCNT/Ni(OH)2 nanocomposite was utilized as the electrochemical interface and modifier of the electrode surface. The simple preparation procedures for MWCNT/Ni(OH)2 composite were provided. Its characteristics and properties were investigated by HRTEM, FESEM, XRD, and FTIR techniques. Leveraging the unique electrochemical characteristics shown by the MWCNT/Ni(OH)2 nanocomposite and its correlation with CEA, high accuracy in CEA detection was achieved. Experimental findings provide evidence that the proposed immunosensor has the ability to detect CEA in laboratory samples. This research contributes towards achieving precise and rapid CEA detection in cancer diagnosis and prognosis. Across a wide concentration range of CEA, the designed immunosensor demonstrated a linear response from 0.0001 ng/mL to 2 ng/mL, and its limit of detection (LOD) was just 0.076 pg/mL. To evaluate the practical applicability of the electrochemical immunosensor, blood serum samples were examined, revealing the immunosensor's remarkable specificity and longevity. Its high accuracy and stability make it a valuable tool in clinical settings and biomedical research, paving the way for improved cancer management and patient outcomes.

Keywords

• Cancer
• CEA
• Biomarker
• Immunosensor
• Ni(OH)2
• MWCNT