Effect of the <i>Croton rhamnifolioides</i> Essential Oil and the Inclusion Complex (OEFC/β-CD) in Antinociceptive Animal Models
Anita Oliveira Brito Pereira Bezerra Martins,
Maria Rayane Correia de Oliveira,
Isabel Sousa Alcântara,
Lindaiane Bezerra Rodrigues,
Francisco Rafael Alves Santana Cesário,
Maria Sanadia Alexandre da Silva,
Fyama Ferreira e Castro,
Emmily Petícia do Nascimento,
Thaís Rodrigues de Albuquerque,
Lucindo José Quintans Júnior,
Adriano Antunes de Souza Araújo,
Henrique Douglas Melo Coutinho,
Irwin Rose Alencar de Menezes,
Almir Gonçalves Wanderley
Affiliations
Anita Oliveira Brito Pereira Bezerra Martins
Laboratory of Pharmacology and Preclinical Bioactive Product Toxicology, Graduate Program in Pharmaceutical Sciences, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235—Cidade Universitária, Recife 50670-901, Brazil
Maria Rayane Correia de Oliveira
Laboratory of Pharmacology and Molecular Chemistry, Department of Biological Chemistry, Regional University of Cariri, Rua Coronel Antônio Luis 1161, Pimenta, Crato 63105-000, Brazil
Isabel Sousa Alcântara
Laboratory of Pharmacology and Molecular Chemistry, Department of Biological Chemistry, Regional University of Cariri, Rua Coronel Antônio Luis 1161, Pimenta, Crato 63105-000, Brazil
Lindaiane Bezerra Rodrigues
Laboratory of Pharmacology and Molecular Chemistry, Department of Biological Chemistry, Regional University of Cariri, Rua Coronel Antônio Luis 1161, Pimenta, Crato 63105-000, Brazil
Francisco Rafael Alves Santana Cesário
Laboratory of Pharmacology and Molecular Chemistry, Department of Biological Chemistry, Regional University of Cariri, Rua Coronel Antônio Luis 1161, Pimenta, Crato 63105-000, Brazil
Maria Sanadia Alexandre da Silva
Laboratory of Pharmacology and Molecular Chemistry, Department of Biological Chemistry, Regional University of Cariri, Rua Coronel Antônio Luis 1161, Pimenta, Crato 63105-000, Brazil
Fyama Ferreira e Castro
Laboratory of Pharmacology and Molecular Chemistry, Department of Biological Chemistry, Regional University of Cariri, Rua Coronel Antônio Luis 1161, Pimenta, Crato 63105-000, Brazil
Emmily Petícia do Nascimento
Laboratory of Pharmacology and Molecular Chemistry, Department of Biological Chemistry, Regional University of Cariri, Rua Coronel Antônio Luis 1161, Pimenta, Crato 63105-000, Brazil
Thaís Rodrigues de Albuquerque
Laboratory of Pharmacology and Molecular Chemistry, Department of Biological Chemistry, Regional University of Cariri, Rua Coronel Antônio Luis 1161, Pimenta, Crato 63105-000, Brazil
Lucindo José Quintans Júnior
Laboratory of Neuroscience and Pharmacological Assays, Department of Physiology, Federal University of Sergipe, Avenue Marechal Rondon, S/N, São Cristóvão 49100-000, Brazil
Adriano Antunes de Souza Araújo
Laboratory of Pharmaceutical Testing and Toxicity, Department of Pharmacy, Federal University of Sergipe, Avenida Marechal Rondon, S/N, São Cristóvão 49100-000, Brazil
Henrique Douglas Melo Coutinho
Laboratory of Microbiology and Molecular Biology, Department of Biological Chemistry, Regional University of Cariri, Rua Coronel Antônio Luis 1161, Pimenta, Crato 63105-000, Brazil
Irwin Rose Alencar de Menezes
Laboratory of Pharmacology and Molecular Chemistry, Department of Biological Chemistry, Regional University of Cariri, Rua Coronel Antônio Luis 1161, Pimenta, Crato 63105-000, Brazil
Almir Gonçalves Wanderley
Laboratory of Pharmacology and Preclinical Bioactive Product Toxicology, Graduate Program in Pharmaceutical Sciences, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235—Cidade Universitária, Recife 50670-901, Brazil
This study aims to evaluate the antinociceptive effect of the C. rhamnifolioides leaf essential oil (OEFC) and the β-cyclodextrin inclusion complex (COEFC) and investigate the pain signaling pathways involved in the antinociceptive response. The effects of the OEFC and COEFC on the central nervous system (CNS) were determined by open field and rota-rod assays, and the antinociceptive effect was evaluated via the acetic acid-induced abdominal contortions, formalin, and hot plate models. Swiss (Mus musculus) male mice (20–30 g) were used in both trials. The OEFC (200 mg/kg/v.o-orally) and COEFC (83.5 mg/kg/v.o.) did not present alterations in the CNS. The OEFC (25, 50, 100, and 200 mg/kg/vo.) and COEFC (8.35, 41.75, and 83.5 mg/kg/v.o.) demonstrated antinociceptive effects in the abdominal contortions, formalin, and hot plate tests. The OEFC (25 mg/kg/v.o.) and COEFC (8.35 mg/kg/v.o.) doses showed that the antinociceptive effect involves the activation of the opioid, cholinergic, and vanilloid systems, as well as the L-arginine/NO and α-2 adrenergic receptor pathways. The antinociceptive potential the OEFC and COEFC demonstrate possible alternatives for the therapy of pain. However, the COEFC presented more significant effects at lower doses than the isolated OEFC, where this action may be justified by the properties and advantages of the complexation.
