Macromol (Apr 2021)

Effect of the <i>Croton rhamnifolioides</i> Essential Oil and the Inclusion Complex (OEFC/β-CD) in Antinociceptive Animal Models

  • Anita Oliveira Brito Pereira Bezerra Martins,
  • Maria Rayane Correia de Oliveira,
  • Isabel Sousa Alcântara,
  • Lindaiane Bezerra Rodrigues,
  • Francisco Rafael Alves Santana Cesário,
  • Maria Sanadia Alexandre da Silva,
  • Fyama Ferreira e Castro,
  • Emmily Petícia do Nascimento,
  • Thaís Rodrigues de Albuquerque,
  • Lucindo José Quintans Júnior,
  • Adriano Antunes de Souza Araújo,
  • Henrique Douglas Melo Coutinho,
  • Irwin Rose Alencar de Menezes,
  • Almir Gonçalves Wanderley

DOI
https://doi.org/10.3390/macromol1020008
Journal volume & issue
Vol. 1, no. 2
pp. 94 – 111

Abstract

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This study aims to evaluate the antinociceptive effect of the C. rhamnifolioides leaf essential oil (OEFC) and the β-cyclodextrin inclusion complex (COEFC) and investigate the pain signaling pathways involved in the antinociceptive response. The effects of the OEFC and COEFC on the central nervous system (CNS) were determined by open field and rota-rod assays, and the antinociceptive effect was evaluated via the acetic acid-induced abdominal contortions, formalin, and hot plate models. Swiss (Mus musculus) male mice (20–30 g) were used in both trials. The OEFC (200 mg/kg/v.o-orally) and COEFC (83.5 mg/kg/v.o.) did not present alterations in the CNS. The OEFC (25, 50, 100, and 200 mg/kg/vo.) and COEFC (8.35, 41.75, and 83.5 mg/kg/v.o.) demonstrated antinociceptive effects in the abdominal contortions, formalin, and hot plate tests. The OEFC (25 mg/kg/v.o.) and COEFC (8.35 mg/kg/v.o.) doses showed that the antinociceptive effect involves the activation of the opioid, cholinergic, and vanilloid systems, as well as the L-arginine/NO and α-2 adrenergic receptor pathways. The antinociceptive potential the OEFC and COEFC demonstrate possible alternatives for the therapy of pain. However, the COEFC presented more significant effects at lower doses than the isolated OEFC, where this action may be justified by the properties and advantages of the complexation.

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