MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences,, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Szeged, Hungary
Gergely Komlósi
MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences,, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Szeged, Hungary
Gáspár Oláh
MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences,, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Szeged, Hungary
Judith Baka
MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences,, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Szeged, Hungary
Gábor Molnár
MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences,, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Szeged, Hungary
MTA-SZTE Research Group for Cortical Microcircuits of the Hungarian Academy of Sciences,, Department of Physiology, Anatomy and Neuroscience, University of Szeged, Szeged, Hungary
Summation of ionotropic receptor-mediated responses is critical in neuronal computation by shaping input-output characteristics of neurons. However, arithmetics of summation for metabotropic signals are not known. We characterized the combined ionotropic and metabotropic output of neocortical neurogliaform cells (NGFCs) using electrophysiological and anatomical methods in the rat cerebral cortex. These experiments revealed that GABA receptors are activated outside release sites and confirmed coactivation of putative NGFCs in superficial cortical layers in vivo. Triple recordings from presynaptic NGFCs converging to a postsynaptic neuron revealed sublinear summation of ionotropic GABAA responses and linear summation of metabotropic GABAB responses. Based on a model combining properties of volume transmission and distributions of all NGFC axon terminals, we predict that in 83% of cases one or two NGFCs can provide input to a point in the neuropil. We suggest that interactions of metabotropic GABAergic responses remain linear even if most superficial layer interneurons specialized to recruit GABAB receptors are simultaneously active.
