Small RNA Profiling in Mycobacterium Provides Insights Into Stress Adaptability

Yingyu Chen; Yingyu Chen; Wenjun Zhai; Wenjun Zhai; Kailun Zhang; Kailun Zhang; Han Liu; Han Liu; Tingting Zhu; Tingting Zhu; Li Su; Li Su; Luiz Bermudez; Huanchun Chen; Huanchun Chen; Aizhen Guo; Aizhen Guo

doi:10.3389/fmicb.2021.752537

Frontiers in Microbiology (Nov 2021)

Small RNA Profiling in Mycobacterium Provides Insights Into Stress Adaptability

Yingyu Chen,
Yingyu Chen,
Wenjun Zhai,
Wenjun Zhai,
Kailun Zhang,
Kailun Zhang,
Han Liu,
Han Liu,
Tingting Zhu,
Tingting Zhu,
Li Su,
Li Su,
Luiz Bermudez,
Huanchun Chen,
Huanchun Chen,
Aizhen Guo,
Aizhen Guo

Affiliations

Yingyu Chen: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
Yingyu Chen: National Animal Tuberculosis Para-Reference Laboratory (Wuhan) of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, China
Wenjun Zhai: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
Wenjun Zhai: National Animal Tuberculosis Para-Reference Laboratory (Wuhan) of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, China
Kailun Zhang: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
Kailun Zhang: National Animal Tuberculosis Para-Reference Laboratory (Wuhan) of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, China
Han Liu: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
Han Liu: National Animal Tuberculosis Para-Reference Laboratory (Wuhan) of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, China
Tingting Zhu: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
Tingting Zhu: National Animal Tuberculosis Para-Reference Laboratory (Wuhan) of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, China
Li Su: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
Li Su: National Animal Tuberculosis Para-Reference Laboratory (Wuhan) of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, China
Luiz Bermudez: Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR, United States
Huanchun Chen: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
Huanchun Chen: National Animal Tuberculosis Para-Reference Laboratory (Wuhan) of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, China
Aizhen Guo: State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China
Aizhen Guo: National Animal Tuberculosis Para-Reference Laboratory (Wuhan) of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, China

DOI: https://doi.org/10.3389/fmicb.2021.752537
Journal volume & issue: Vol. 12

Abstract

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Mycobacteria encounter a number of environmental changes during infection and respond using different mechanisms. Small RNA (sRNA) is a post-transcriptionally regulatory system for gene functions and has been investigated in many other bacteria. This study used Mycobacterium tuberculosis and Mycobacterium bovis Bacillus Calmette-Guérin (BCG) infection models and sequenced whole bacterial RNAs before and after host cell infection. A comparison of differentially expressed sRNAs using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) and target prediction was carried out. Six pathogenically relevant stress conditions, growth rate, and morphology were used to screen and identify sRNAs. From these data, a subset of sRNAs was differentially expressed in multiple infection groups and stress conditions. Many were found associated with lipid metabolism. Among them, ncBCG427 was significantly downregulated when BCG entered into macrophages and was associated with increased biofilm formation. The reduction of virulence possibility depends on regulating lipid metabolism.

Published in Frontiers in Microbiology

ISSN: 1664-302X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Science: Microbiology
Website: http://www.frontiersin.org/journals/microbiology

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