Quantificação simultânea de indicadores biológicos de exposição a solventes orgânicos por cromatografia líquida de alta eficiência Simultaneous quantification of organic solvent biomarkers by high performance liquid chroma tography

Abstract

Biological monitoring is very important to guarantee health to workers. This method was developed for simultaneous determination of xylene, toluene, styrene and ethylbenzene metabolites. It involves only dilution and centrifugation of urine samples and improved chromatographic conditions. Analyses show recovery > 95%; r² > 0.99; intermediate precision CV% < 6% and % bias < ±10. Exposed subjects presented at least three metabolites in urine. The method proved to be feasible, reliable and important in biological monitoring, especially in exposure to organic solvent mixtures.

Keywords

• HPLC
• organic solvents
• biological exposure index