Optimization of purification process of lutein from Marigold by macroporous resin

Abstract

Objective: This study aimed to improve the purity of lutein in Marigold. Methods: Lutein was extracted from marigold by sunflower seed oil microemulsion. The crude extract of lutein was saponified with KOH-C2H5OH solution and further purified with macroporous resin. Results: In the static test, HP-20 macroporous resin had the best adsorption and resolution effect of lutein. 80% ethanol solution was used as the adsorption solvent, and anhydrous ethanol solution was used as the analytical solvent, with the loading mass concentration of 0.01 mg/mL. The optimal static adsorption conditions were 30 ℃ temperature, adsorption for 2.0 h, at pH 7.0; the optimal static analytical conditions were temperature 40 ℃, analytical time 1.0 h, at pH 7.0. In the dynamic test, the optimal loading concentration was 0.01 mg/mL, and the loading flow rate was 0.4 mL/min. The purity of lutein after adsorption by macroporous resin reached 61.72%. Conclusion: The purity of lutein in marigold after macroporous resin purification is obviously improved.

Keywords

• marigold
• lutein
• saponification
• column chromatography
• microemulsion