The genus Cucurbita has been mentioned as a good source of carotenoids, including β-carotene, which is a precursor of vitamin A. However, the variability observed for the genus denotes the individual need to invest in the species. An experiment was carried out with the aim of evaluating the content of total carotenoids and β-carotene in C. maxima germplasm. Treatments consisted of 23 accessions and two commercial cultivars of C. maxima. The content of total carotenoids, β-carotene, and the pulp color of the accessions were evaluated. Clustering was performed, and the components of genotypic variance, mean heritability, and accuracy were calculated. The grouping led to the formation of four groups where the total carotenoid content influenced the formation the most. There was no difference in color parameters. High variability was observed for total carotenoids in the studied genotypes (22.28 µg/g to 49.58 µg/g) and β-carotene (7.81 µg/g to 13.75 µg/g). Accessions CMAX-09, CMAX-10, CMAX-12, CMAX-13, and CMAX-14 showed the highest levels of total carotenoids and β-carotene.
