Characteristics and antibiotic resistance patterns of urinary tract isolates in hospitalized and non-hospitalized patients: a cross-sectional study in Khartoum, Sudan

Abstract

Abstract Background Urinary tract infections (UTIs) are widespread worldwide impacting society, economics, and healthcare systems. The rise of antibiotic resistance in both hospital and community settings has further complicated UTI management. This study aims to assess the epidemiologic distribution and microbiologic classification of UTI-causing microorganisms, as well as their resistance patterns. Methods This cross-sectional descriptive study was conducted at the National Public Health Laboratories in Khartoum, Sudan. Urine samples were collected from three hospitals from September to December 2021, and 65 bacterial isolates were recovered from patients with clinical symptoms of UTI. Bacterial identification and antimicrobial susceptibility testing were performed using standard bacteriological methods and Kirby-Bauer’s disc diffusion method. Genotyping for Extended Spectrum Beta-Lactamase (EsβL) production was conducted using multiplex PCR. Results Of the 65 bacterial isolates, 27 (41.5%) were community-acquired UTI patients (C-UTIs), and 38 (58.5%) were hospital acquired UTI patients (H-UTIs). Males were more commonly affected by H-UTIs (68.4%), while females represented the majority among C-UTIs (85.2%). Gram-negative bacteria were predominant in urinary isolates (81.54%), with Escherichia coli being the most common organism (49.1%). Gram-positive organisms accounted for 18.46% of isolates, with Enterococcus spp. being the most prevalent. In-vitro susceptibility analysis revealed that meropenem was the most effective antibiotic in Gram-negative isolates (77.4%), while nitrofurantoin was the most effective in Gram-positive isolates (91.67%). EsβL production was detected in 11/23 (47.8%) of the tested isolates with Escherichia coli being the most common ESβL producer. Conclusion This study highlights the pattern of antibiotic-resistant bacteria in UTIs and their significance. Continuous monitoring, development of local antibiograms, and implementation of surveillance programs are essential for guiding empirical antibiotic use and managing UTIs effectively, especially in resource-limited settings like Sudan.

Keywords

• Urinary tract infections
• Antibiotic resistance
• Escherichia coli
• Meropenem
• Extended Spectrum Beta-Lactamase