Construction of AIEgens and graphene oxide based aptasensor with two-fluorescent signals for determination of Aflatoxin B1 in food Samples
Yongmei Jia,
Qiuxia Lai,
Zhiguo Li,
Guohua Zhou,
Peilian Liu,
Xunbo Lu,
Mingyue Xue,
Yong Cheng
Affiliations
Yongmei Jia
School of Chemistry and Chemical Engineering, Key Laboratory of Clean Energy Materials Chemistry of Guangdong Higher Education Institutes, Lingnan Normal University, Zhanjiang 524048, Guangdong, PR China; Corresponding author: Prof. Yongmei Jia, Lingnan Normal University, School of Chemistry and Chemical Engineering, Zhanjiang 524048, China.
Qiuxia Lai
School of Chemistry and Chemical Engineering, Key Laboratory of Clean Energy Materials Chemistry of Guangdong Higher Education Institutes, Lingnan Normal University, Zhanjiang 524048, Guangdong, PR China
Zhiguo Li
School of Chemistry and Chemical Engineering, Key Laboratory of Clean Energy Materials Chemistry of Guangdong Higher Education Institutes, Lingnan Normal University, Zhanjiang 524048, Guangdong, PR China
Guohua Zhou
School of Chemistry and Chemical Engineering, Key Laboratory of Clean Energy Materials Chemistry of Guangdong Higher Education Institutes, Lingnan Normal University, Zhanjiang 524048, Guangdong, PR China
Peilian Liu
School of Chemistry and Chemical Engineering, Key Laboratory of Clean Energy Materials Chemistry of Guangdong Higher Education Institutes, Lingnan Normal University, Zhanjiang 524048, Guangdong, PR China
Xunbo Lu
School of Chemistry and Chemical Engineering, Key Laboratory of Clean Energy Materials Chemistry of Guangdong Higher Education Institutes, Lingnan Normal University, Zhanjiang 524048, Guangdong, PR China
Mingyue Xue
School of Chemistry and Chemical Engineering, Key Laboratory of Clean Energy Materials Chemistry of Guangdong Higher Education Institutes, Lingnan Normal University, Zhanjiang 524048, Guangdong, PR China
Yong Cheng
Department of NanoEngineering, University of California, San Diego, La Jolla, CA 92093, USA; Corresponding author: Dr. Yong Cheng, Department of NanoEngineering, University of California, San Diego, La Jolla, CA 92093, USA.
Sensitive and accurate determination of aflatoxin B1 in food samples is urgently required for food safety, environment monitoring and human health due to its high toxicity. Here, we constructed an aptasensor with two fluorescent signals for determination of AFB1 in food samples by combining AIEgesns (TPE-Z) as fluorescent probe, the highly specific aptamer molecular label with Cy5 as the recognition element and GO as the fluorescence quencher. Upon the addition of AFB1, the fluorescence signal of the aptasensor platform turn from “off” to “on” with two fluorescent signals. The aptasensor provided a sensitivity (0.04 ng/mL), specificity and reliable approach for AFB1 detection. It had distinct advantage in detection sensitivity compared with single fluorescent signal aptasensor. Moreover, it was applied to analysis AFB1 in food samples (rice, corn and soybean) with satisfactory results.
