Safety Considerations in 3D Bioprinting Using Mesenchymal Stromal Cells

Lucy Belk; Lucy Belk; Nazzar Tellisi; Nazzar Tellisi; Nazzar Tellisi; Hamish Macdonald; Ahmet Erdem; Ahmet Erdem; Ahmet Erdem; Ahmet Erdem; Nureddin Ashammakhi; Nureddin Ashammakhi; Nureddin Ashammakhi; Nureddin Ashammakhi; Nureddin Ashammakhi; Ippokratis Pountos; Ippokratis Pountos; Ippokratis Pountos

doi:10.3389/fbioe.2020.00924

Frontiers in Bioengineering and Biotechnology (Oct 2020)

Safety Considerations in 3D Bioprinting Using Mesenchymal Stromal Cells

Lucy Belk,
Lucy Belk,
Nazzar Tellisi,
Nazzar Tellisi,
Nazzar Tellisi,
Hamish Macdonald,
Ahmet Erdem,
Ahmet Erdem,
Ahmet Erdem,
Ahmet Erdem,
Nureddin Ashammakhi,
Nureddin Ashammakhi,
Nureddin Ashammakhi,
Nureddin Ashammakhi,
Nureddin Ashammakhi,
Ippokratis Pountos,
Ippokratis Pountos,
Ippokratis Pountos

Affiliations

Lucy Belk: Academic Department of Trauma and Orthopaedics, University of Leeds, Leeds, United Kingdom
Lucy Belk: School of Medicine, University of Leeds, Leeds, United Kingdom
Nazzar Tellisi: Academic Department of Trauma and Orthopaedics, University of Leeds, Leeds, United Kingdom
Nazzar Tellisi: School of Medicine, University of Leeds, Leeds, United Kingdom
Nazzar Tellisi: Chapel Allerton Hospital, Leeds Teaching Hospitals, Leeds, United Kingdom
Hamish Macdonald: Gloucester Royal Hospital, Gloucestershire Hospitals NHS Foundation Trust, Gloucester, United Kingdom
Ahmet Erdem: Center for Minimally Invasive Therapeutics, University of California, Los Angeles, Los Angeles, CA, United States
Ahmet Erdem: Department of Bioengineering, Henry Samueli School of Engineering, University of California, Los Angeles, Los Angeles, CA, United States
Ahmet Erdem: Department of Chemistry, Kocaeli University, Kocaeli, Turkey
Ahmet Erdem: Department of Biomedical Engineering, Kocaeli University, Kocaeli, Turkey
Nureddin Ashammakhi: Center for Minimally Invasive Therapeutics, University of California, Los Angeles, Los Angeles, CA, United States
Nureddin Ashammakhi: Department of Bioengineering, Henry Samueli School of Engineering, University of California, Los Angeles, Los Angeles, CA, United States
Nureddin Ashammakhi: Department of Radiological Sciences, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA, United States
Nureddin Ashammakhi: 0Institute for Quantitative Health Science and Engineering, Michigan State University, East Lansing, MI, United States
Nureddin Ashammakhi: 1Department of Biomedical Engineering, Michigan State University, East Lansing, MI, United States
Ippokratis Pountos: Academic Department of Trauma and Orthopaedics, University of Leeds, Leeds, United Kingdom
Ippokratis Pountos: School of Medicine, University of Leeds, Leeds, United Kingdom
Ippokratis Pountos: Chapel Allerton Hospital, Leeds Teaching Hospitals, Leeds, United Kingdom

DOI: https://doi.org/10.3389/fbioe.2020.00924
Journal volume & issue: Vol. 8

Abstract

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Three-dimensional (3D) bioprinting has demonstrated great potential for the fabrication of biomimetic human tissues and complex graft materials. This technology utilizes bioinks composed of cellular elements placed within a biomaterial. Mesenchymal stromal cells (MSCs) are an attractive option for cell selection in 3D bioprinting. MSCs can be isolated from a variety of tissues, can pose vast proliferative capacity and can differentiate to multiple committed cell types. Despite their promising properties, the use of MSCs has been associated with several drawbacks. These concerns are related to the ex vivo manipulation throughout the process of 3D bioprinting. The herein manuscript aims to present the current evidence surrounding these events and propose ways to minimize the risks to the patients following widespread expansion of 3D bioprinting in the medical field.

Published in Frontiers in Bioengineering and Biotechnology

ISSN: 2296-4185 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology: Biotechnology
Website: http://www.frontiersin.org/bioengineering_and_biotechnology

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