The effectiveness of biogenic silver nanoparticles in the treatment of caprine mastitis induced by Staphylococcus aureus

Abstract

The aim of the present study is to determine the efficiency of silver nanoparticles in mastitis treatment induced by Staphylococcus aureus, 20 lactating goats were infected intramammaryinoculated with 1.5×108 cfu/ml of virulence Staphylococcus aureus isolated from milk of caprine mastitis and at 48hr post inoculation, the animals were divided randomly into four groups equally, 1st group left without treatment as control positive, 2nd group treated intramammaryinfusion with 5 ml Silver nanoparticle after 2h from infection daily for 3 consecutive days, 3rd group treated intramammaryinfusion with 5 ml Silver nanoparticle after 72h from infection daily for 3 consecutive days, 4th group treated intramammaryinfusion with 5 ml Silver nanoparticle mixed with gentamycin after 72h from infection daily for 3 consecutive days. The silver nanoparticles used in this study were created using a green method from Bacillus clausii. The Scanning electron microscope was spherical and homogeneous form, with a size range of 25 to 45 nm. Results showed severe clinical signs of the mammary gland with heavy bacterial isolation in 1st control groups at 3, 6-, 9-, 14- and 21-days post infection, while animals that were intramammary treated with Silver nanoparticle alone or combination with gentamycin showed a disappearance in clinical signs of the mammary gland and bacterial isolation, it was concluded that Silver nanoparticle had a strong action against mastitis caused by Staphylococcus aureus.

Keywords

• silver nanoparticle
• milk
• mastitis
• staphylococcus aureus